Human marrow stromal cell lines which sustain hematopoieses

Details Human marrow stromal cell lines which sustain hematopoieses Human marrow stromal cell lines which sustain hematopoieses

1997-05-23

1999-03-09

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Culture medium, per se

435 701, 435325, 435366, 435372, 435405, L12N 500

Patent

active

058799400

ABSTRACT:
Immortalized human stromal cell lines sustain and expand human hematopoietic precursor cells. The precursor cells are obtained from a blood product and inoculated into a culture medium conditioned by exposure to a human stromal cell line. Preferred human stromal cell lines secrete SCF, LIF, MIP1.alpha., and IL-6, as exemplified by a human stromal cell line designated HS-1. The conditioned culture medium may be supplemented with additional growth factors, such as interleukin-3. After expansion the human hematopoietic precursor cells are harvested and returned to a patient or frozen and stored. The immortalized human stromal cell lines can also be used as feeder layers in ex vivo bone marrow cultures or in colony forming assays.

REFERENCES:
patent: 5004681 (1991-04-01), Boyse et al.
patent: 5215927 (1993-06-01), Berenson et al.
patent: 5322787 (1994-06-01), Martin et al.
Galy et al., "Untransfected and SV40-transfected fetal and postnatal human thymic stromal cells", Thymus, 22:13-33 (1993).
Nuttal et al., "Gene Expression in `Immortalized` Human Marrow Stromal Stem Cell-Lines", J. Bone & Mineral Res., 7(S1):PS201 (1992).
Lanotte et al., "Histochemical and Ultrastructural Characteristics of a Cell Line form Human Bone Marrow Stroma," J. Cell Sci. 50:281 (1981).
Zipori et al., "Cultured Mouse Marrow Cell Lines: Interactions Between Fibroblastoid Cells and Monocytes," J. Cell Physiol. 118:143 (1984).
Song et al., "Radioresistant Murine Marrow Stromal Cells: A Morphologic and Functional Characterization," Exp. Hematol. 12:523 (1984).
Zipori et al., "Cultured Mouse Marrow Stromal Cell Lines, II. Distinct Sybtypes Differing in Morphology Collage Types, Myelopoietic Factors, and Leukemic Cell Growth Modulating Activities," J. Cell Physiol. 122:81 (1985).
Harigaya et al., "Generation of Functional Clonal Cell Lines from Human Bone Marrow Stroma," Proc. Natl. Acad. Sci. USA 82:3477 (1985).
Tsai et al., "Isolation of a Human Stromal Cell Strain Secreting Hemopoieteic Growth Factors," J. Cell Physiol. 127:137 (1986).
Singer et al., "Simian Virus 40-Transformed Adherent Cells from Human Long-term Marrow Cultures: Clones Cell Lines Produce Cells with Stromal and Hematopoietic Characteristics," Blood 70:464 (1987).
Novotny et al., "Cloned Stromal Cell Lines Derived from Human Whitlock/Witte-type Long-term bone Marrow Cultures," Exp. Hematol. 18:775 (1990).
Slack et al., "Regulation of Cytokine and Growth Factor Gene Expression in human Bone Marrow Stromal Cells Transformed with Simian Virus 40, " Blood 75:2319 (1990).
Charbord et al., "The Cytoskeleton of Stromal Cells from Human Bone Marrow Cultures REsembles that of Cultured Smooth Muscle Cells," Exp. Hematol. 18:276 (1990).
Dorshkind, "Regulation of Hemopoiesis by Bone Marrow Stromal Cells and their Products," Ann. Rev. Immunol. 8:111 (1990).
Sutherland et al., "Functional Characterization of Individual Human Hematopoietic Stem Cell Cultured at Limiting Dilution on Supportive Marrow Stromal Layers," Proc. Natl. Acad. Sci. USA 87:3584 (1990).
Eaves et al., "Mechanisms that Regulate the Cell Cycle Status of Very Primative Hematopoietic cells in Long-Term Human Marrow Cultures. II. Analysis of Positive and Negative Reuglators Produced by Stromal Cells within the Adherent Layer," Blood 78: 100 (1991).
Simmons et al., "Identification of Stromal cell Precursors in Human Bone Marrow by a Novel Monoclonal Antibody, STRO-1," Blood 78: 55-62 (1991).
Eaves et al., "Methodology of Long-Term Culture of Human Hemopoietic Cells," J. Cult. Meth.. 13:55 (1991).
Halbert et al., "The E7 Gene of Human Papillomavirus Type 16 is Sufficient for Immortalization of Human Epithelial Cells," J. Virol. 65:473 (1991).
Kittler et al., "Biologic Significance of Constitutive and Subliminal Growth Factor Production of Bone Marrow Stroma," Blood 79: 3168 (1992).
Quesenberry, "Stroma-Dependent Hematolymphopoietic Stem Cells," Curr. Topics Microbiol. Immunol. 177: 151 (1992).
Berenson et al. "Transplantation of Stem Cells Enriched by Immunoadsorption," (Adv. Bone Marrow Purging & Processings, N.Y.: Wiley-Liss, p. 449, (1992).
Cicutinni et al., "Support of Human Cord Blood Progenitor Cells on Human Stromal Cell Lines Transformed by SV.sub.40 Large T Antigen under the Influence of an Inducible (Metallothionein) Promoter," Blood 80: 102 (1992).
Clark et al., "Cell Adhesion in the Stromal Regulation of Haemopoiesis," Bailliere's Clin. Haematol. 5: 619 (1992).
Heinrich et al., "Constitutive Expression of Steel Factor Gene by Human Stromal Cells,," Blood 82: 771 (1993).
Ogawa, "Differentiation and Proliferation of Hematopoietic Stem Cells," Blood 81: 2844 (1993).
Deryugina, et al., "Stromal Cells in Long-Term Cultures: Keys to the Elucidation of Hematopoietic Development?" Critical Rev. Immunol. 82:66 (1993).
Galmiche, et al., "Stromal Cells from Human Long-Term Marrow Cultures are Mesenchymal Cells that Differentiate Following a Vascular Smooth Muscle Differentiation Pathway," Blood, 82:66 (1993).
Thalmeir et al., "Establishment of Two Permanent Human Bone Marrow Stromal Cell Lines with Long-Term Post Irradiation Feeder Capacity," Blood 83: 1799 (1994).
Ryan, et al., "HK-2: An Immortalized Proximal Tubule Epithelial Cell Line from Normal Adult Human Kidney," Kidney Int. 45:48 (1994).
Verfaille, Blood 82(7):2045-2053 (1993).
Fibbe et al., Lumphokine Res., 7(3):290 (1988).
Rinehart et al., Proc. Am. Assoc. Cancer Res. Annu. Meet., 34(0):148 (1993).
Weber et al., J. Cell. Biochem., Suppl. 13C:48 (1989).
Chiang et al., Blood 84 (10 Suppl 1 ):418A (1994).
Roecklein et al., Blood 85(4): 997-1005 (1995).

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