Human lymphoblastold cell line and hybridomas derived therefrom

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435240, 435948, 935100, C12N 1500, C12N 500, C12R 191

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046249214

ABSTRACT:
A 6-thioguanine-resistant subvariant of the EBV-transformed human lymphoblastoid B cell line WI-L2 is described. The subvariant line, designated LTR228, fuses efficiently with human cells. Human.times.human hybridomas derived from LTR228 that produce monoclonal antibodies against tetanus toxin and blood group A are exemplified.

REFERENCES:
patent: 4451570 (1984-05-01), Royston et al.
Kozbor et al, "Human Hybridomas Constructed with Antigen-Specific Epstein-Barr Virus Transformed Cell Lines", Proceedings of the National Academy of Sciences, 79, pp. 6651-6655 (1982).
Physicians' Desk Reference, p. 1382, Litton Industries 31st Ed. (1977).
Choy et al, "Techniques for Using Hat Selection in Somatic Cell Genetics", Techniques in Somatic Cell Genetics, Ed. Shay, Plenum Press, NY, pp. 11-21 (1982).
Chiorazzi, N., et al., J. Exp. Med. (1982) 156:930-935.
Handley, H. H., et al, Proceedings of the 15th International Leucocyte Culture Conference, Asilomar (1982), p. 267.

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