Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1992-05-01
1996-06-25
Chambers, Jasemine C.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
424 92, 424 931, 435 4, 435 721, 435 6, 435 691, 43524021, 4351723, 4352403, 43524031, 4353201, 935 55, 935 57, C12N 506, C12N 510, C12Q 102, A01N 6300
Patent
active
055299202
ABSTRACT:
The present invention relates to long term multiplication and permanent establishment of a cell line of human liver epithelial cells(hepatocytes). The human liver epithelial cell line is capable of mitotically proliferating and continuously growing in vitro under suitable environmental conditions in suitable culture media. A method of producing an immortalized human liver epithelial cell line is also disclosed. The invention also relates to serum-free cell medium developed to support long term multiplication and permanent establishment of a cell line of human liver epithelial cells. The medium may contain an effective cell growth promoting amount of calcium ions; an effective cell growth promoting amount of glucose; an effective amount of insulin to aid cells in glucose uptake; an effective cell growth promoting amount of hydrocortisone; an effective amount of epidermal growth factor to bind epidermal growth factor receptors on cells; an effective amount of transferrin to increase DNA synthesis in cells; an effective amount of cholera toxin to increase DNA synthesis in cells; an effective amount of triiodothyronine to increase DNA synthesis in cells; and an effective growth promoting amount of mammalian hormones and mitogenic factors, including lipoprotein, cholesterol, phospholipids and fatty acids.
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Cole Katharine H.
Harris Curtis C.
Lechner John F.
Pfeifer Andrea M.
Reddel Roger
Chambers Jasemine C.
Stanton Brian R.
The United States of America as represented by the Secretary of
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