Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1996-07-16
1998-08-04
Sisson, Bradley L.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 6, 435 912, 935 8, C07H 2104, C12P 1934, C12N 1500
Patent
active
057895686
ABSTRACT:
The present invention provides a method for detection of at least one allele of a genetic locus and can be used to provide direct determination of the haplotype. The method comprises amplifying genomic DNA with a primer pair that spans an intron sequence and defines a DNA sequence in genetic linkage with an allele to be detected. The primer-defined DNA sequence contains a sufficient number of intron sequence nucleotides to characterize the allele. Genomic DNA is amplified to produce an amplified DNA sequence characteristic of the allele. The amplified DNA sequence is analyzed to detect the presence of a genetic variation in the amplified DNA sequence such as a change in the length of the sequence, gain or loss of a restriction site or substitution of a nucleotide. The variation is characteristic of the allele to be detected and can be used to detect remote alleles. Kits comprising one or more of the reagents used in the method are also described.
REFERENCES:
New England BioLabs 1988-1989 Catalog, p. 62, 1988.
Search Report for nucleotide residue sequence "CTCTCAGGCCTTGTT", Dec. 1996.
GeneType A.G.
Sisson Bradley L.
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