Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Patent
1997-04-02
1999-03-23
Ketter, James
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
C12N 508
Patent
active
058858326
ABSTRACT:
A human lens epithelial cell line capable of expressing an exogeneous immortalizing gene, which is established without infecting with a live virus. A method for producing the human lens epithelial cell line, which comprises transfecting human lens epithelial cells with an expression vector functionally carrying an exogeneous immortalizing gene, and subculturing said cells in a medium. The above-mentioned human lens epithelial cell line wherein the cells proliferate in a temperature sensitive manner. A method for producing a temperature sensitive human lens epithelial cell line, which comprises transfecting human lens epithelial cells with an expression vector functionally carrying an exogeneous immortalizing gene, and subculturing said cells in a medium. Since the human lens epithelial cell line of the present invention is free of infection with a live virus, the cells are useful as biomaterials. Moreover, since the temperature sensitive human lens epithelial cell line of the present invention stops proliferation at about 36.degree. C. or above, it may be used as a biomaterial for regenerating a human lens.
REFERENCES:
patent: 5514552 (1996-05-01), Rosner et al.
patent: 5529920 (1996-06-01), Cole et al.
patent: 5643782 (1997-07-01), Andley et al.
U. Andley et al., Investigative Ophthalmology & Visual Science, 35(7), 3094-3102 (1994).
Ketter James
Senju Pharmaceutical Co. Ltd.
Yucel Irem
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