Human keratinocytes supported on a hydrophilic membrane and meth

Drug – bio-affecting and body treating compositions – Preparations characterized by special physical form – Implant or insert

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Details

424422, 424423, 424424, 424443, 514928, 623 15, A61F 210, A61F 1300

Patent

active

056933320

ABSTRACT:
The present invention provides keratinocytes supported on a hydrophilic membrane, the composition being useful as a skin graft to effect closure of a wound. The invention also provides methods of preparing a keratinocyte-containing membrane suitable for effecting wound closure. In addition, the invention provides methods of effecting closure of wound comprising contacting the wound with a keratinocyte-containing membrane.

REFERENCES:
patent: 5326356 (1994-07-01), Della Valle et al.
Barlow, Y.M. et al., "The Use of a Polymeric Film for the Culture and Transfer of Sub-confluent Autologous Keratinocytes to Patients." J. Tissue Viability. 2:33-36 (1992).
Hansbrough, John F. et al., "Evaluation of Graftskin.star-solid. Compositie Grafts on Full-Thickness Wounds on Athymic Mice." J. Burn Care & Rehabilitation. l0 15:346-353 (1994).
Hansbrough, John F. and Cooper, Matthew L., "Methods of Skin Coverage." Critical Care Report. 2:48-62 (1990).
Hansbrough, John F. et al., "Development of a Temporary Living Skin Replacement Composed of Human Neonatal Fibroblasts Cultured in Biobrane, a Synthetic Dressing Material." Surgery. 115:633-644 (1994).
Hansbrough, John F. et al., "Composite Grafts of Human Keratinocytes Grown on a Polyglactin Mesh-Cultured Fibroblasts Dermal Substitute Function as a Bilayer Skin Replacement in Full-Thickness Wounds on Athymci Mice." J. Burn Care & Rehabilitation. 14:485-494 (1993).

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