Human jagged polypeptide, encoding nucleic acids and methods of

Details Human jagged polypeptide, encoding nucleic acids and methods of Human jagged polypeptide, encoding nucleic acids and methods of

1997-06-25

2000-10-24

Spector, Lorraine

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

15 to 23 amino acid residues in defined sequence

530300, 530327, 530350, 536 235, 536 231, 435372, C07K 700, C07K 14475

Patent

active

061369527

ABSTRACT:
The present invention provides an isolated polypeptide exhibiting substantially the same amino acid sequence as JAGGED, or an active fragment thereof, provided that the polypeptide does not have the amino acid sequence of SEQ ID NO:5 or SEQ ID NO:6. The invention further provides an isolated nucleic acid molecule containing a nucleotide sequence encoding substantially the same amino acid sequence as JAGGED, or an active fragment thereof, provided that the nucleotide sequence does not encode the amino acid sequence of SEQ ID NO:5 or SEQ ID NO:6. Also provided herein is a method of inhibiting differentiation of hematopoietic progenitor cells by contacting the progenitor cells with an isolated JAGGED polypeptide, or active fragment thereof. The invention additionally provides a method of diagnosing Alagille Syndrome in an individual. The method consists of detecting an Alagille Syndrome disease-associated mutation linked to a JAGGED locus.

REFERENCES:
patent: 5869282 (1999-02-01), Ish-Horowicz et al.
Baker et al., "Spacing Differentiation in the Developing Drosophila Eye: A Fibrinogen-Related Lateral Inhibitor Encoded by scabrous," Science 250:1370-1377 (1990).
Corbin et al., "A Role for the Drosophila Neurogenic Genes in Mesoderm Differentiation," Cell 67:311-323 (1991).
Cuoso and Arias, "Notch is required for wingless Signaling in the Epidermis of Drosophila, " Cell 79:259-272 (1994).
Deleuze et al., "Mapping of Microsatellite Markers in the Alagille Region and Screening of Microdeletions by Genotyping 23 Patients," Eur. J. Hum. Genet. 2:185-190 (1994).
Ellisen et al., "TAN-1, the Human Homolog of the Drosophila Notch Gene, Is Broken by Chromosomal Translocations in T Lymphoblastic Neoplasms," Cell 66:649-661 (1991).
Fuller et al., "Making Decisions: Role of Hematopoietic Growth Factors in Lineage Commitment and Maturation," Hematopoiesis Keystone Symposia (1997).
GenBank accession U61276 and statement from GenBank User Services regarding date of first public release (Jul. 23, 1996).
GenBank accession U77720 and statement from GenBank User Services regarding date of first public release (Nov. 25, 1996).
GenBank accession U77914 and statement from GenBank User Services regarding date of first public release (Nov. 25, 1996).
Henderson et al., "lag-2 may encode a signaling ligand for the GLP-1 and LIN-12 Receptors of C. elegans," Development 120:2913-2924 (1994).
Krantz et al., "Alagille syndrome," J. Med. Genet. 34:152-157 (1997).
Krantz et al., "Deletions of 20p12 in Alagille Syndrome: Frequency and Molecular Characterization," Am. J. Med. Genet. 70:80-86 (1997).
Li et al., "Alagille syndrome is caused by mutations in human Jagged1, which encodes a ligand for Notch1," Nature Genetics 16:243-251 (1997).
Li et al., "The Human Homolog of Rat Jagged1 Expressed by Marrow Stroma Inhibits Differentiation of 32D cells through Interaction with Notch1," Immunity 8:43-55 (1998).
Lindsell et al., "Jagged: A Mammalian Ligand That Activates Notch1," Cell 80:909-917 (1995).
McAdams et al., "Hematopoietic cell culture therapies (Part 1): cell culture considerations," Trends in Biotech. 14:341-349 (1996).
Milner et al., "A Human Homologue of the Drosophila Developmental Gene, Notch, Is Expressed in CD34.sup.+ Hematopoietic Precursors," Blood 83(8):2057-2062 (1994).
Milner et al., "Inhibition of granulocytic differentiation by mNotch1," Proc. Natl. Acad. Sci. 93:13014-13019 (1996).
Nye and Kopan, "Vertebrate ligands for Notch," Current Biology 5(9):966-969 (1995).
Nye et al., "An activated Notch suppresses neurogenesis and myogenesis but not gliogenesis in mammalian cells," Development 120:2421-2430 (1994).
Oda et al., "Mutations in the human Jagged1 gene are responsible for Alagille syndrome," Nature Genetics 16:235-242 (1997).
Pollet et al., "Construction of a 3.7-Mb Physical Map within Human Chromosome 20p12 Ordering 18 Markers in the Alagille Syndrome Locus," Genomics 27:467-474 (1995).
Rand et al., "Molecular Analysis of 24 Alagille Syndrome Families Identifies a Single Submicroscopic Deletion and Further Localizes the Alagille Region within 20p12," Am. J. Hum. Gene. 57:1068-1073 (1995).
Robey et al., "An Activated Form of Notch Influences the Choice Between CD4 and CD8 T Cell Lineages," Cell 87:483-492 (1996).
Roecklein and Torok-Storb, "Functionally Distinct Human Marrow Stromal Cell Lines Immortalized by Transduction with the Human Papilloma Virus E6/E7 Genes," Blood 85(4):997-1005 (1995).
Schnittger et al., "Molecular and cytogenetic analysis of an Interstitial 20p deletion associated with syndromic intrahepatic ductular hypoplasia (Alagille syndrome)," Hum. Genet. 83:239-244 (1989).
Shawber et al., "Jagged2: A Serrate-like Gene Expressed during Rat Embryogenesis," Dev. Biol. 180:370-376 (1996).
Simpson, "The Notch connection," Nature 375:736-737 (1995).
Spinner et al., "Cytologically Balanced t(2;20) in a Two-Generation Family with Alagille Syndrome: Cytogenetic and Molecular Studies," Am. J. Hum. Genet. 55:238-243 (1994).
Tax et al., "Sequence of C. elegans lag-2 reveals a cell-signaling domain shared with Delta and Serrate of Drosophilia," Nature 368:150-154 (1994).
Thiemann et al., "Retroviral Transduction of CD34+ Subpopulations on AFT024 Stroma," Hematopoiesis Keystone Symposia #419 (1997).
Zimrin et al., "An Antisense Oligonucleotide to the Notch Ligand Jagged Enhances Fibroblast Growth Factor-induced Angiogenesis in Vitro," J. Biol. Chem. 271(51):32499-32502 (1996).
Oda et al., "Identification and cloning of the human homolog (JAG1) of the rat Jagged1 gene fro the Alagille syndrome critical region at 20p12," Genomics 43(3):376-379 (1997).

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