Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal
Patent
1982-10-29
1984-04-03
Hazel, Blondel
Chemistry of carbon compounds
Miscellaneous organic carbon compounds
C-metal
424 85, 435 68, 435811, C07G 700, A61K 4502
Patent
active
044406754
ABSTRACT:
Disclosed is a process for the purification of crude immune interferon to a near homogeneous preparation which comprises: (a) adsorbing the crude interferon onto a column containing Controlled Pore Glass beads and eluting with ammonium sulfate, (b) adsorbing the interferon containing eluant onto a column containing either Concanavalin A-Sepharose, lentil lectin-Sepharose or pea lectin-agarose and eluting with a buffer containing a sugar, (c) adsorbing the interferon containing eluant onto a column containing Heparin-Sepharose or Procian Red-agarose and eluting with a high salt content buffer, (d) adsorbing the interferon containing eluant onto a cationic exchange resin column and eluting with a salt buffer and (e) treating the interferon containing eluant in a gel-filtration column equilibrated in high salt to obtain a solution of immune interferon that is nearly homogeneous.
REFERENCES:
patent: 4314935 (1982-02-01), Uemura et al.
Langford, M., et al., Injection and Immunity, vol. 26, pp. 36-41, 1979.
Yip et al., Proc. Natl. Acad. Sci., vol. 78, pp. 1601-1605, 1981.
O'Malley, Methods in Enzymology, vol. 78, pp. 540-545, Academic Press, 1981.
Chemical Abstracts, vol. 92, p. 590, Abst No. 39568r, 1980.
Chemical Abstracts, vol. 91, p. 49, Abst No. 209059z, 1979.
Chemical Abstracts, vol. 93, Abst No. 130300d, 1980.
Hazel Blondel
Meloy Laboratories Inc.
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