Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1990-09-21
1995-06-13
Parr, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 29, 4351723, 4352401, 4352402, 436501, 436 92, 436815, 436816, 536 231, 530350, 935 6, 935 9, 935 78, C12Q 168, C07H 2104
Patent
active
054241855
ABSTRACT:
Non-primate or primate cells are provided comprising a functional human transporter for neurotransmitter uptake. The cells allow for dissection of the mechanism of neurotransmitter transport, as well as screening for agonists and antagonists of the neurotransmitter with respect to its uptake. Methods are provided for producing such cells. Specifically, the cells are transformed with human DNA comprising the gene encoding for the neurotransmitter transporter, whereby this protein(s) is expressed and incorporated into the plasma membrane and is capable of functioning to transfer the neurotransmitter from the extracellular space to intracellular domains. The physiological, kinetic and pharmacological characteristics of transport in these cells conform to known characteristics of high-affinity neurotransmitter transport in the CNS.
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Chang Albert S.
Lam Dominic M.
Baylor College of Medicine
Marschel Ardin H.
Parr Margaret
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