Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-07
1998-12-29
Ulm, John
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435243, 4353201, 435325, 435455, 435471, 530399, 530402, 536 2351, C12N 1518, C12N 1563, C07K 1461
Patent
active
058540261
ABSTRACT:
A human growth hormone variant including the set of amino acid substitutions H18A, Q22A, F25A, D26A, Q29A, E65A, K168A, E174A is disclosed. Also disclosed is a nucleic acid encoding this variant, along with a vector including the nucleic acid, a host cell including the vector, and a process for preparing the variant. The variant has enhanced affinity for human growth hormone receptor at site 1.
REFERENCES:
patent: 3853832 (1974-12-01), Li
patent: 3853833 (1974-12-01), Li
patent: 4446235 (1984-05-01), Seeburg
patent: 4665160 (1987-05-01), Seeburg
patent: 4670393 (1987-06-01), Seeburg
patent: 4699897 (1987-10-01), Jones et al.
patent: 4871832 (1989-10-01), Aviv et al.
patent: 4880910 (1989-11-01), deBoer et al.
patent: 4888286 (1989-12-01), Crea
patent: 5223409 (1993-06-01), Ladner et al.
patent: 5350836 (1994-09-01), Kopchick et al.
Queen, C. et al., "A humanized antibody that binds to the interleukin 2 receptor", Proc. Natl. Acad. Sci. USA, 86:10029-10033 (1989).
Argos, P., "An Investigation of Protein Subunit and Domain Interfaces", Protein Eng. 2:101-113 (1988).
Aston, R. et al., "Monoclonal Antibodies to Growth Hormone and Prolactin", Pharmac. Ther. 27:403-424 (1985).
Barany, G. et al., "Solid-Phase Peptide Synthesis" The Peptides 2:3-254 (Gross & Meienhofer, eds., Academic Press 1979).
Bass, S. et al., "Hormone Phage: An Enrichment Method for Variant Proteins With Altered Binding Properties", Proteins: Struct., Funct., Genet. 8:309-314 (1990).
Baumann, G. et al., "A Specific Growth Hormone-Binding Protein in Human Plasma: Initial Characterization", Journal of Clinical Endocrinology and Metabolism 62:134-141 (1986).
Carter, P. et al., "Improved Oligonucleotide Site-directed mutagenesis using M13 vectors", Nucl. Acids Res. 13:4431-4443 (1986).
Chawla, R. et al., "Structural Variants of Human Growth Hormone: Biochemical, Genetic, and Clinical Aspects", App. Rev. Med. 34:519-547 (1983).
Chothia, C., "The Nature of the Accessible and Buried Surfaces in Proteins", J. Mol. Biol. 105:1-12 (1976).
Cunningham, B. et al., "Dimerization of the Extracellular Domain of the Human Growth Hormone Receptor by a Single Hormone Molecule", Science 254:821-825 (1991).
Cunningham, B. et al., "Rational design of receptor-specific variants of human growth hormone", Proc. Natl. Acad. Sci. USA 88:3407-3411 (1991).
Davies, D. et al., "Antibody-Antigen Complexes", Ann. Rev. Biochem. 59:439-473 (1990).
De Vos, A. et al., "Human Growth Hormone and Extracellular Domain of Its Receptor: Crystal Structure of the Complex", Science 255:306-312 (1992).
Edwards, C. et al., "A Newly Defined Property of Somatotropin: Priming of Macrophages for Production of Superoxide Anion", Science 239:769-771 (1988).
Fuh, G. et al., "Rational Design of Potent Antagonists to the Human Growth Hormone Receptor", Science 256:1677-1680 (1992).
Geysen, H. et al., "Use of Peptide Synthesis to Probe Viral Antigens for Epitopes to a Resolution of a Single Amino Acid", Proc. Natl. Acad. Sci. USA 81:3998-4002 (1984).
Herington, A. et al., "Identification and Characterization of Specific Binding Proteins for Growth Hormone in Normal Human Sera", J. Clin. Invest. 77:1817-1823 (1986).
Hughes, J. et al., "The Nature and Regulation of the Receptors for Pituitary Growth Hormone", Ann. Rev. Physiol. 47:469-482 (1985).
Isaksson, O. et al., "Mode of Action of Pituitary Growth Hormone on Target Cells", Ann. Rev. Physiol. 47:483-499 (1985).
Janin, J. et al., "Surface, Subunit Interfaces and Interior of Oligomeric Proteins", J. Mol. Biol. 204:155-164 (1988).
Li, C. et al., "Human Pituitary Growth Hormone. XII. The Amino Acid Sequence of the Hormone", J. Am. Chem. Soc. 88:2050-2051 (1966).
Lowman, H. et al., "Selecting High-Affinity Binding Proteins by Monovalent Phage Display", Biochemistry 30:10832-10838 (1991).
Lowman, H. et al., "Monovalent Phage Display: A Method for Selecting Variant Proteins from Random Libraries", Methods: Companion Methods Enzymol. 3:205-216 (1991).
Mandel, M. et al., "Calcium-dependent Bacteriophage DNA Infection", J. Mol. Biol. 53:159-162 (1970).
Martial, J. et al., "Involvement of Lysine Residues in the Binding of hGH and bGH to Somatrotropic Receptors", FEBS Lett. 180:295-299 (1985).
Miller, S., "The Structure of Interfaces Between Subunits of Dimeric and Tetrameric Proteins", Protein Eng. 3:77-83 (1989).
Nicoll, C. et al., "Structural Features of Prolactins and Growth Hormones That Can Be Related to Their Biological Properties", Endocrine Reviews 7:169-203 (1986).
Paladini, A. et al., "Molecular Biology of Growth Hormone", CRC Crit. Rev. Biochem. 15:25-56 (1983).
Roberts, B. et al., "Directed evolution of a protein: Selection of potent neutrophil elastase inhibitors displayed on M13 fusion phage", Proc. Natl. Acad. Sci. USA 89:2429-2433 (1992).
Rutter, W. et al., "Redesigning Proteins via Genetic Engineering", Protein Engineering 257-267 (Oxender & Fox, eds., A.R. Liss, Inc. 1987).
Thorner, M. et al., "Growth Hormone, 1988", J. Clin. Invest. 82:745-747 (1988).
Wells, J. et al., "Importance of Hydrogen-Bond Formation in Stabilizing the Transition State of Subtilisin", Phil. Trans. R. Soc. Lond. Ser. A 317:415-423 (1986).
Wells, J., "Additivity of Mutational Effects in Proteins", Biochem. 29:8509-8517 (1990).
Wells, J. et al., "Structure and Function of Human Growth Hormone: Implications for the Hematopoietins", Ann. Rev. Biophys. Biomol. Struct. 22:329-351 (1993).
Zoller, M. et al., "Oligonucleotide-directed Mutagenesis Using M13-derived Vectors: An Efficient and General Procedure for the Production of Point Mutations in any Fragment of DNA", Nuc. Ac. Res. 10:6487-6500 (1982).
Abdel-Maeguid et al., "Three-Dimensional Structure of a Genetically Engineered Variant of Porcine Growth Hormone", Proc. Natl. Acad. Sci. USA 84:6434-6437 (1987).
Armstrong et al., "Domain Structure of Bacteriophage fd Adsorption Protein", FEBS Letters 135: 167-172 (1981).
Bajt et al., Characterization of a Gain of Function Mutation of Integrin .alpha.II.beta.3 (Platelet Glycoprotein IIb-IIIa), J. Biol. Chem. 267:22211-22216 (1992).
Barlow et al., "Continous and Discontinuous Protein Antigenic Determinants", Nature 322:747-748 (1986).
Bennett et al., "High Resolution Analysis of Functional Determinants on Human Tissue-Type Plasminogen Activator", J. Biol. Chem. 266:5191-5201 (1991).
Berendt et al., "The Binding Site on ICAM-1 for Plasmodium Falciparum-Infected Erythrocytes Overlaps, But is Distinct LFA-1-Binding Site", Cell 68:71-81 (1992).
Berlot et al., "Identification of Effector-Activating Residues of G s.alpha.", Cell 68:911-9822 (1992).
Bettler et al, "Immunoglobulin in E-Binding Site in Fc Receptor (FcRII/CD23) Identified by Homolog-Scanning Mutagenesis", J. Biol. Chem. 267:185-191 (1992).
Boutin et al., "Cloning and Expression of the Rat Prolactin Receptor, a Member of the Growth Hormone/Prolactin Receptor Gene Family", Cell 53:69-77 (1988).
Bowie et al., "Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions", Science 247:1306-1310 (1990).
Burstein et al., "Immunoreactivity and Receptor Binding of Mixed Recombinants of Human Growth Hormone and Chorionic Somatomammotropin", Proc. Natl. Acad. Sci. USA 75:5391-5394 (1978).
Camble et al., "Properties of Interferon .alpha.2 Analogures Produced from Synthetic Genes" in Peptides: Structure and Function, Proceedings of the 9th American Peptide Symposium 375-384, Deber et al., eds., Pierce Chemical Co., Chicago, IL (1985).
Chang et al., "High-Level Secretion of Human Growth Hormone by Escherichia coli", Gene 55:189-196 (1987).
Clayton et al., "Substitution of Murine for Human CD4 Residues Identifies Amino Acids Critical for HIV-gp120 Binding", Nature 355:363-366 (1988).
Crissman and Smith, "Gene-III Protein of Filamentous Phages: Evidence for a Carboxyl-Terminal Domain with a Role in Morphogenesis", Virology 132:445-455 (1984).
Cunningham et al., "Engineering Human Prolactin to Bind to the Human Growth Hormone Receptor", Science 24:1461-1465 (1990).
Cunningham, et al., "High-Resolution Epitope Mapping of hGH-Receptor Interaction by Alanine-Scanning Mutagenesis", Scie
Cunningham Brian C.
Wells James A.
Genentech Inc.
Saoud Christine
Ulm John
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