Human glutaredoxin .beta.

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

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4353201, 435325, 435 691, 435 6, 536 232, 536 235, C12N 910, C12N 1585, C12N 1500, C12Q 168

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059687983

ABSTRACT:
The invention provides a human glutaredoxin .beta. (GRX.beta.) and polynucleotides which identify and encode GRX.beta.. The invention also provides expression vectors, host cells, agonists, antibodies and antagonists. The invention also provides methods for treating disorders associated with expression of GRX.beta..

REFERENCES:
Padilla, C.A. et al., "High-level expression of fully active human glutaredoxin (thioltransferase) in E. coli and characterization of Cys.sup.7 to Ser mutant protein." FEBS Lett. (1996) 378:69-73. (GI 531405).
Schallreuter, K.U. and J.M. Wood, "New aspects in the pathophysiology of cutaneous melanoma: a review fo the role of thioproteins and the effect of nitrosoureas." Melanoma Research (1991) 1:159-167.
Jung, C.H.and J.A. Thomas, "S-glutathiolated Hepatocyte Proteins and Insulin Disulfides as Substrates for Reduction by Glutaredoxin, Thioredoxin, Protein Disulfide Isomerase, and Glutathione." Arch.Biochem.Biophys. (1996) 335:61-72.
Park, J.B. et al., "Purification, cloning and expression of dehydroascorbic acid-reducing activity from human neutrophils: identification as glutaredoxin." Biochem.J. (1996) 315:931-938.
Tsang, M.L. J. Weatherbee, "Thioredoxin, glutaredoxin, and thioredoxin reductase from cultured HeLa cells." Proc.Natl.Acad.Sci.USA (1981) 78:7478-7482.
Szerderkenyi, J. et al., (GI 1628431) GenBank Sequence Database (Accession Z49699), National Center for Biotechnology Information: National Library of Medicine, Bethesda, Maryland 20849. (GI 1732424) (Sep. 8, 1997).
Gan, Z.R. "Cloning and Sequencing of a Gene Encoding Yeast Thioltransferase." Biochem.Biophys.Res.Commun. (1992) 187:949-955.
Schallreuter, K.U. et al., "The mechanism of action of the nitrosourea anti-tumor drugs on thioredoxin reductase, glutathione reductase and ribonucleotide reductase." Biochem.Biophys.Acta (1990) 1054:14-20.
Padilla, A.C., (GI 531404) GenBank Sequence Database (Accession X76648), National Center for Biotechnology Information: National Library of Medicine, Bethesda, Maryland 20849. (GI 531405) (Jan. 10, 1996).
Hillier, L. et al., WashU-Merck EST Project, Accession: AA056193, Jul. 18, 1996.
Park, J. et al., Biochem. J., vol. 315, pp. 931-938, May 16, 1996.

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