Human glucocorticoid receptor 1A promoter and splice variants

Details Human glucocorticoid receptor 1A promoter and splice variants Human glucocorticoid receptor 1A promoter and splice variants

2007-09-11

2007-09-11

Nickol, Gary B. (Department: 1646)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S023100, C435S069100, C435S320100, C435S252300, C435S325000

Reexamination Certificate

active

10620641

ABSTRACT:
A new sequence, hGR 1Ap/e, has been isolated from human DNA upstream from the previously known 2.7 kbp human GR promoter region. This new sequence was found to contain a new promoter (the 1A GR promoter) and a new untranslated exon sequence (GR exon 1A) for the human glucocorticoid receptor protein (Hgr). Alternative splicing produces three different hGR 1A-containing transcripts, 1A1, 1A2, and 1A3. Exon 1A3-containing GR transcripts appear to be restricted to blood cell cancers and to the human brain. Glucocorticoid hormone treatment caused an up-regulation of exon 1A3-containing GR transcripts in T-lymphoblast cells, and a down-regulation of exon 1A3-containing transcripts in B-lymphoblast cells. Thus detection of exon 1A3-containing transcripts can be used for the diagnosis of patients with blood cell cancers, including T-cell acute lymphoblastic leukemia (ALL), and to identify patients that would benefit from glucocorticoid hormone treatment.

REFERENCES:
The Journal Of Biological Chemistry, vol. 266, No. 11, Apr. 15, 1991, pp. 7182-7188.
Amara, S.G. et al., “Alternative RNA Processing in Calcitonin Gene Expression Generates mRNAs Encoding Different Polypeptide Products,”Nature, vol. 298, pp. 240-244 (1982).
Bradshaw, H.D., Jr. and W.V. Vedeckis, “Glucocorticoid Effects on Thymidine Incorporation into the DNA of S49 Lymphoma Cells,” J. Steroid Biochem., vol. 18, pp. 691-698 (1983).
Breslin, M.B. and W. V. Vedeckis, “The Glucocorticoid Receptor and c-junPromoters Contain AP-1 Sites that Bind Different AP-1 Transcription Factors,”Endocrine, vol. 5, pp. 15-22 (1996).
Breslin, M.B. and W. V. Vedeckis, “The Human Glucocorticoid Receptor Promoter Upstream Sequences Contain Binding Sites for the Ubiquitous Transcription Factor, Yin Yang 1,”J. Steroid Biochem. Molec. Biol., vol. 67, pp. 369-381 (1998).
Chen et al., “Association of the Glucocorticoid Receptor Alternatively-Spliced Transcript 1A with the Presence of the High Molecular Weight Membrane Glucocorticoid Receptor in Mouse Lymphoma Cells,”J. Cell. Biochem., vol. 74, pp. 430-446, (1999b).
Chen et al., “Multiple Glucocorticoid Receptor Transcripts in Membrane Glucocorticoid Receptor-Enriched S-49 Mouse Lymphoma Cells,”J. Cell. Biochem., vol. 74, pp. 418-429 (1999a).
DeAngelis, M.M. , “Assembly of a High-Resolution May of the Acadian Usher Syndrome Region and Localization of the Human Nuclear EF-Hand Acidic Gene,” Biochim. Biophys. Acta, vol. 1407, pp. 84-91 (1998.
Denton, R.R. et al., “Differential Autoregulation of Glucocorticoid Receptor Expression in Human T- and B-Cell Lines,”Endocrinology, vol. 133, pp. 248-256 (1993).
Distelhorst, C.W., “Basic and Clinical Studies of Glucocorticosteroid Receptors in Lymphoid Malignancies,” pp. 494-515 in W. V. Vedeckis (ed.)Hormones and Cancer(1996).
Dong et al., “Regulation of Glucocorticoid Receptor Expression: Evidence for Transcriptional and Posttranslational Mechanisms,”Mol. Endocrinol., vol. 2, pp. 1256-1264 (1988).
Eisen, L. P. et al., “Positive Regulation of the Glucocorticoid Receptor in Human T-Cells Sensitive to the Cytolytic Effects of Glucocorticoids,”J. Biol. Chem., vol. 263, pp. 12044-12048 (1988).
Encio, I.J. and S.D. Detera-Wadleigh, “The Genomic Structure of the Human Glucocorticoid Receptor,”J. Biol. Chem., vol. 266, pp. 7182-7188 (1991).
Ferguson, A.T. et al., Demethylation of the Estrogen Receptor Gene in Estrogen Receptor-Negative Breast Cancer Cells Can Reactivate Estrogen Receptor Gene Expression,Cancer Res., vol. 55, pp. 2279-2283 (1995).
Gasson, J.C. et al., “A New Determinant of Glucocorticoid Sensitivity in Lymphoid Cell Lines,”J. Cell. Biol., vol. 96, pp. 409-415 (1983.
Geley, S. et al., Resistance to Glucocorticoid-Induced Apoptosis in Human T-Cell Acute Lymphoblastic Leukemia CEM-C1 cells is Due to insufficient Glucocorticoid Receptor Expression.Cancer Res., vol. 56, pp. 5033-5038 (1996).
Gomi, M. et al., “Glucocorticoid Effects on Myeloma Cells in Culture: Correlation of Growth Inhibition with Induction of Glucocorticoid Receptor Messenger RNA,”Cancer Res., vol. 50, pp. 1873-1878, (1990).
Grabowski, P.J., “Splicing Regulation in Neurons: Tinkering with Cell-Specific Control,”Cell, vol. 92, pp. 709-712, (1998).
Günzburg, W. H. et al., “Regulated Gene Expression After Retroviral Vector-Mediated Delivery of Cancer-Relevant Therapeutic Genes,”Recent Results Cancer Res., vol. 144, pp. 116-126 (1998).
Harada, H. et al., “Structurally Similar but Functionally Distinct Factors, IRF-1 and IRF-2, Bind to the Same Regulatory Elements of IRFN and IFN-Inducible Genes,”Cell, vol. 58, pp. 729-739 (1989).
Hollenberg et al., “Primary Structure and Expression of a Functional Human Glucocorticoid Receptor cDNA,”Nature, vol. 318, pp. 635-641 (1985).
Jonas, V. et al., Alternative RNA Processing Events in the Human Calcitonin/Calcitonin Gene-Related Peptide Gene Expression,Proc. Natl. Acad. Sci. USA, vol. 82, pp. 1994-1998 (1985).
Kalinyak, J.E.., “Tissue-specific Regulation of Glucocorticoid Receptor mRNA by Dexamethasone,”J. Biol. Chem., vol. 262, pp. 10441-10444 (1987).
Kroll, R.A. et al., “Outwitting the Blood-Brain Barrier for Therapeutic Purposes: Osmotic Opening and Other Means,”Neurosurgery, vol. 42, pp. 1083-1099 (1998).
Lapidus, R.G. et al., “Methylation of Estrogen and Progesterone Receptor 5′ CpG Islands Correlates with Lack of Estrogen and Progesterone Receptor Gene Expression in Breast Tumors,”Clin. Cancer Res., vol. 2, pp. 805-810 (1996).
Murayama, Y. et al., “Cell-Specific Expression of the Diphtheria Toxin A-Chain Coding Sequence Under the Control of the Upstream Region of the Human Alpha-Fetoprotein Gene,”J. Surg. Oncol., vol. 70, 145-149 (1999).
Nobukuni, Y. et al., “Characterization of the Human Glucocorticoid Promoter,”Biochemistry, vol. 34, pp. 8207-8214 (1995).
Norman, M.R. and E. B. Thompson, “Characterization of a Glucocorticoid-Sensitive Human Lymphoid Cell Line,” Cancer Res., vol. 37, pp. 3785-3791 (1977).
Okret, S. et al., “Down-Regulation of Glucocorticoid Receptor mRNA by Glucocorticoid Hormones and Recognition by the Receptor of a Specific Binding Sequence Within a Receptor cDNA Clone,”Proc. Natl. Acad. Sci. USA, vol. 83, pp. 5899-5903 (1986).
Ottaviano, Y.L. et al., “Methylation of the Estrogen Receptor CpG Island Marks Loss of Estrogen Receptor Expression in Human Breast Cancer Cells,”Cancer Res., vol. 54, pp. 2552-2555 (1994).
Ramdas, J. et al., “Glucocorticoid-Induced Cell Death Requires Autoinduction of Glucocorticoid Receptor Expression in Human Leukemic T Cells,”Cancer Res., vol. 59, pp. 1378-1385 (1999).
Robertson, M.W., III, et al., “Use of a Tissue-Specific Promoter for Targeted Expression of the Herpes Simplex Thymidine Kinase Gene in Cervical Carcinoma Cells,”Cancer Gene Ther., vol. 5, pp. 331-336 (1998).
Roskrow, M.A. et al., “Recent Developments in Gene Therapy for Oncology and Hematology,”Crit. Rev. Oncol. Hematol., vol. 28, pp. 139-151 (1998).
Sharp, P.A., “Split Genes and RNA Splicing,”Cell, vol. 77, pp. 805-815 (1994).
Sparmann, G. et al., “Conditional Expression of Human TNF-alpha: a System for Inducible Cytotoxicity,”Int. J. Cancer, vol. 59, pp. 103-107 (1994).
Stevens, J. et al., “Characterization of Cytosolic and Nuclear Glucocorticoid-Binding Components in Human Leukemic Lymphocytes,”Cancer Res., vol. 39, pp. 4939-4948 (1979).
Strähle et al., “At Least Three Promoters Direct Expression of the Mouse Glucocorticoid Receptor Gene,”Proc. Natl. Acad. Sci. USA., vol. 89, pp. 6731-6735 (1992).
Tanaka, N. et al., “Recognition DNA Sequences of Interferon Regulatory Factor 1 (IRF-1) and IRF-2, Regulators of Cell Growth and the Interferon System,”Mol

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