Human FAS

Details Human FAS Human FAS

1999-03-03

2001-09-25

LeGuyader, John L. (Department: 1633)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

C435S252300, C435S320100, C435S325000, C536S023100, C536S023200, C536S023500

Reexamination Certificate

active

06294364

ABSTRACT:

FIELD OF THE INVENTION
This invention relates to newly identified polypeptides and polynucleotides encoding such polypeptides, to their use in therapy and in identifying compounds which may be agonists, antagonists and/or inhibitors which are potentially useful in therapy, and to production of such polypeptides and polynucleotides.
BACKGROUND OF THE INVENTION
The drug discovery process is currently undergoing a fundamental revolution as it embraces ‘functional genomics’, that is, high throughput genome- or gene-based biology. This approach is rapidly superseding earlier approaches based on ‘positional cloning’. A phenotype, that is a biological function or genetic disease, would be identified and this would then be tracked back to the responsible gene, based on its genetic map position.
Functional genomics relies heavily on the various tools of bioinformatics to identify gene sequences of potential interest from the many molecular biology databases now available. There is a continuing need to identify and characterise further genes and their related polypeptides/proteins, as targets for drug discovery.
While the pathway for the biosynthesis of saturated fatty acids is similar in prokaryotes and eukaryotes, the organization of the biosynthetic apparatus is very different. Vertebrates and yeast possess fatty acid synthases (FASs) in which all of the enzymatic activities are encoded on one or two polypeptide chains, respectively, and the acyl carrier protein (ACP) is an integral part of the complex. In contrast, in most bacterial FASs each of the reactions are catalyzed by distinct monofunctional enzymes and the ACP is a discrete protein. There therefore is considerable potential for selective inhibition of the bacterial systems by broad spectrum antibacterial agents.
SUMMARY OF THE INVENTION
The present invention relates to Fatty Acid Synthase, in particular Fatty Acid Synthase polypeptides and Fatty Acid Synthase polynucleotides, recombinant materials and methods for their production. In another aspect, the invention relates to methods for using such polypeptides and polynucleotides, including the treatment of cancers and bacterial infections, hereinafter referred to as “the Diseases”, amongst others. In a further aspect, the invention relates to methods for identifying agonists and antagonists/inhibitors using the materials provided by the invention, and treating conditions associated with Fatty Acid Synthase imbalance with the identified compounds. In a still further aspect, the invention relates to diagnostic assays for detecting diseases associated with inappropriate Fatty Acid Synthase activity or levels.


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