Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2004-09-24
2008-08-19
Ton, Thaian N (Department: 1632)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S366000, C435S383000
Reexamination Certificate
active
07413904
ABSTRACT:
This disclosure provides a system for obtaining genetically altered primate pluripotent stem (pPS) cells. The role of the feeder cells is replaced by supporting the culture on an extracellular matrix, and culturing the cells in a conditioned medium. The cells can be genetically altered with a viral vector or DNA/lipid complex, and then selected for successful transfection by drug-resistant phenotype in the transfected cells. The system allows for bulk proliferation of genetically altered pPS cells as important products for use in human therapy or drug screening.
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Carpenter Melissa K.
Gold Joseph D.
Inokuma Margaret S.
Xu Chunhui
Geron Corporation
Mittler E. Stewart
Ton Thaian N
LandOfFree
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