Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Patent
1997-10-14
1999-09-14
Saucier, Sandra E.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
4352351, 435239, C12N 508, C12N 700, C12N 702
Patent
active
059522275
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
The present invention relates to a novel human embryonic lung fibroblast diploid cell strain suitable for producing a virus and a process for the preparation of varicella zoster virus using same as a host cell.
DESCRIPTION OF THE PRIOR ART
Viruses cause various diseases such as measles, rubella, mumps, chickenpox, epidemic hemorrhagic fever, Japanese B encephalitis, infantile poliomyelitis, hepatitis A, hepatitis B, hepatitis C and variola. These viral diseases can be prevented by inoculation with vaccines prepared from inactivated or attenuated pathogenic viruses.
Generally, embryonated chicken eggs, infant rat brain cells and diploid cells of mammals have been used as host cells for producing such virus vaccines. Although embryonated chicken eggs or infant rat brain cells may be used as host cells to reduce the production cost, they are disadvantageous due to their low susceptibility to viruses, complicated purification processes and side effects brought about by the presence of foreign protein contaminants. In contrast, the use of normal diploid cells originating from human can reduce the side effects caused by foreign proteins, and thus, they are more preferred in preparing virus vaccines.
Representative human diploid cells include MRC-5 cell strain(ATCC, CCL 171), WI-38 cell strain(ATCC, CCL 75) and HEL 299 cell strain(ATCC CCL 137): Specifically, MRC-5 cell strain originated from the lung tissue of a SCI. Arts. Zagreb., pp43-45(1970); and Nature(London), 227, pp168-170(1970)!; WI-38 derived from the lung tissue of a 3 month-old p240(1962)!; and HEL 299 cell strain obtained from the lung tissue of a p772(1968)!.
Chickenpox is a highly contagious disease that afflicts children, aged people and patients having reduced immunity due to the infection by varicella zoster virus(VZV). It has been reported that VZV can be cultured in various cells such as a human amnion cell, human thyroid cell, human Meurisse et al., J. Microbiol., 2, 317(1969)!. Further, U.S. Pat. No. 3,985,615 discloses the production of VZV vaccine by multiple attenuation of Oka virus in a guinea pig primary embryonic tissue cell(GPEC); U.S. Pat. No. 4,000,256 describes the production of VZV vaccine by subculturing 10 to 80 times the human embryonic fibroblast WI-38 cell strain containing VZV virus; U.S. Pat. No. 4,000,317 reported the production of temperature-sensitive mutant VZV in WI-38 cell strain; and U.S. Pat. No. 5,360,736 discloses an improved method for the production of VZV vaccine in MRC-5 cell strain.
However, the yield of VZV is very low in GPEC cells. Further, WI-38 and MRC-5 cell strains have high passage numbers, thereby reducing their capability to produce VZV. Therefore, the above method cannot be applied in a large scale production of VZV vaccine. Further, VZV tends to become inactivated in a cell-free environment owing to its cell-dependent properties.
SUMMARY OF THE INVENTION
Accordingly, it is an object of the present invention to provide a novel human diploid cell strain having a high susceptibility to various viruses, especially to VZV and providing a high yield of VZV.
It is another object of the present invention to provide an improved process for producing VZV.
In accordance with one aspect of the present invention, there is provided a fibroblast diploid cell strain LBHEL(KCTC 0127BP) derived from a human embryonic lung cell.
In accordance with another aspect of the present invention, there is also provided a process for producing a cell-free varicella zoster virus (VZV) comprising the steps of: LBHEL(KCTC 0127BP) in a culture vessel to give cultured LBHEL cells; cell-free VZV.
BRIEF DESCRIPTION OF THE DRAWINGS
The above and other objects and features of the present invention will become apparent from the following description thereof, when taken in conjunction with the accompanying drawings wherein:
FIGS. 1--1 and 1-2 show the karyotype of the LBHEL cell strain of the present invention;
FIG. 2 represents the growth curves of: LBHEL cell strain of the present invention(.diamond-s
REFERENCES:
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patent: 5756281 (1998-05-01), Martin
Jacobs et al., "Characterization of a human diploid cell designated MCR-5", Nature, 1970, vol. 227, pp. 168-170.
Hayflick et al., "The Serial cultivation of human diploid cell strains", Experimental Cell Research, 1961, vol. 25, pp. 585-621.
Kim Jeong Min
Park Bock Ryeon
Park Joo Hong
LG Chemical Ltd
Saucier Sandra E.
Vera Afremova
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