Human derived immortalized liver cell line

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se

Reexamination Certificate

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C435S325000

Reexamination Certificate

active

09673958

ABSTRACT:
The present invention relates to a new immortalized hepatocyte culture of human (preferably human fetal) normal cell origin. The immortalized hepatocyte culture of human normal cell origin of the present invention is useful in, for example, screening for compounds or salts thereof having therapeutic/preventive effects on hepatic insufficiency.

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Masayoshi Nanba et al., “A Study of Immortalization of Normal Human Cells Using Chemical Substances and Radiation (Kagaku busshitsu oyobi houshasen ni yoru hito seijou saibou no fushika ni kansuru kenkyuu)”, Research on Drug Innovation Project: Drug Innovation Research Report—Stage I Comprehensive Report, pp. 163-165 (1995) [with English translation].
Masahiro Miyazaki et al. “A Study of Liver Cell Culture and Hepatocarcinogenesis (Kansaibou baiyou to kanhatsugan no kenkyuu)”, Journal of Medical Association of Okayama (Okayama Igakkai Zasshi) vol. 103, No. 3, pp. 337-347 (1991) [with English translation].
Masahiro Miyazaki et al., “Immortalization of Epithelial-like Cells from Human Liver Tissue with SV40 T-Antigen Gene”, Experimental Cell Research vol. 206, No. 1, pp. 27-35 (1993).
Tomokazu Matsuura, “The Way to the Artificial Liver—Advances in Hepatocytes and Bioreactors: Cells Useful for the Artificial Liver -Patenchymal and Non-parenchymal Cells of the Liver- (Jinkou kanzou heno michi—kansaibou to bioreactor no shimpo Jinkoukan ni riyousuru saibou Kanjisshitsu saibou to hijisshitsu saibou)”, The Tissue Culture (Gekkan Soshiki Baiyou Kougaku) vol. 23, No. 8, pp. 288-291 (1997) [with English translation].

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