Human cysteine protease

Details Human cysteine protease Human cysteine protease

1998-11-17

1999-12-28

Guzo, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 536 245, 435325, C12Q 168, L07H 2104, C12N 1585

Patent

active

060079975

ABSTRACT:
The present invention provides a polynucleotide (ncp) which identifies and encodes a novel cysteine protease (NCP) expressed in cells of both the adrenal gland and human umbilical vein endothelium. The present invention also provides for antisense molecules and oligomers designed from the nucleotide sequence or its antisense. The invention further provides genetically engineered expression vectors and host cells for the production of purified NCP peptide, antibodies capable of binding to NCP, inhibitors which bind to NCP and pharmaceutical compositions based on NCP specific antibodies or inhibitors. The invention specifically provides for diagnostic assays based on altered ncp expression and which allow identification of such a condition. These assays utilize probes designed from ncp encoding or controlling nucleic acid sequences or antibodies specific for the NCP.

REFERENCES:
Rojanasakul et al., Antisense oligonucleotidde therapeutics: drug delivery and targeting, Advanced Drug Delivery Reviews, vol. 18, pp. 115-131, 1996.
Gewirtz et al., Facilitating oligonucleotide delivery: helping antisense deliver on its promise, Proc. Natl. Acad. Sci., vol. 93, pp. 3161-3163, Apr. 1996.
Branch, A good antisense molecule is hard to find, TIBS, vol. 23, pp. 45-50, Feb. 1998.
Bouillaud, F., (Direct Submission), GenBank Sequence Database (Accession R17110), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, 20894 (GI 770720), Jul. 11, 1995.
Okubo, K., et al., (Direct Submission), GenBank Sequence Database (Accession No. D12242), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, 20894 (GI 767729), Nov. 18, 1992.
Hillier, L., (Direct Submission), GenBank Sequence Database (Accession R66824), National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, 20894 (GI 839462), Apr. 19, 1995.
Merckelbach, A. et al., "cDNA sequences of Schistosoma japonicum coding for two cathepsin B-like proteins and Sj32", Trop. Med. Parasitol., 45: 193-198 (1994).
Azaryan, A.V. and Vivian Y.H. Hook, "Distinct Properties of Prohormone Thiol Protease (PTP) Compared to Cathepsins B, L, and H: Evidence for PTP as a Novel Cysteine Protease", Arch. Biochem. Biophys., 314: 171-177 (1994).
Schiller, M.R. et al., "`Prohormone Thiol Protease`(PTP) Processing of Recombinant Proenkephalin", Biochemistry, 34: 7988-7995 (1995).

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