Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Fusion protein or fusion polypeptide
Reexamination Certificate
2003-06-17
2009-02-03
Saidha, Tekchand (Department: 1652)
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Fusion protein or fusion polypeptide
C435S232000, C435S252300, C435S320100, C536S023200
Reexamination Certificate
active
07485307
ABSTRACT:
Human cystathionine β-synthase variants are disclosed, as well as a method to produce recombinant human cystathionine β-synthase and variants thereof. More particularly, the role of both the N-terminal and C-terminal regions of human CBS has been studied, and a variety of truncation mutants and modified CBS homologues are described. In addition, a method to express and purify recombinant human cystathionine β-synthase (CBS) and variants thereof which have only one or two additional amino acid residues at the N-terminus are described.
REFERENCES:
patent: 5523225 (1996-06-01), Kraus
patent: 5635375 (1997-06-01), Kraus et al.
patent: 5656425 (1997-08-01), Kraus
patent: WO 95/07714 (1995-03-01), None
Bruno S. et al. Functional Properties of the Active Core of Human Cystathione beta-Synthase Crystals, J. Biol. Chem. 276, 16-19, Jan. 5, 2001.
Janosik M. et al. Crystallization and preliminary X-ray diffraction analysis of the active core of human recombinant cystathione beta-synthase: an enzyme involved in vascular disease, Act Cryst. Feb. 2001, D57, 289-291.
Shan X. et al. Correction of disease-causing CBS mutation in yeast, Nature Genetics, 1998, May 19, 91-93.
Janosik et al., 2001,Biochemistry, 40:10625-10633.
Jhee et al., 2000,J. Biol. Chem., 275(16):11541-11544.
Kery et al., 1998,Arch. Biochem. Biophys., 355(2):222-232.
Meier et al., 2001,EMBO J., 20(15):3910-3916.
Nozaki et al., 2001,J. Biol. Chem. 276(9):6516-6523.
Ojha et al., 2002,Biochemistry, 41:4649-4554.
Shan et al., 2001,Human Mol. Gen., 10(6):635-643.
Taoka and Banerjee, 2001,J. Inorganic Biochem., 87:245-251.
“A novel protease for site-specific cleavage of GST fusion proteins,” Science Tools from Pharmacia Biotech 2(1):18-19 (1997).
Kraus Jan P.
Oliveriusova Jana
Cha Don D.
Hamilton DeSanctis & Cha, LLP
Saidha Tekchand
The Regents of the University of Colorado
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