Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
1998-04-03
2001-04-17
Whisenant, Ethan (Department: 1655)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S023100, C536S024300
Reexamination Certificate
active
06218115
ABSTRACT:
TECHNICAL FIELD
This invention relates to human cyclin I gene. More specifically, it relates to the amino acid sequence for human cyclin I and a polynucleotide encoding the human cyclin I.
BACKGROUND ART AND RELATED APPLICATIONS
Cyclin is a general term to describe polypeptides that are subunits controlling the activities of cyclin-dependant protein kinase (Cdk) and eight species of cyclin, namely cyclins A~H, have been documented. Cyclin is known to form a complex with Cdk and to exhibit the capability of intracelluar phosphorylation.
Also, structural characteristics common in cyclins are that they posses a region called “cyclin box” which comprises about 100 amino acids within portions of their amino acid sequences. It is recognized that the eight species of cyclins hitherto known are provided with a high degree of homology in the amino acid sequences of this cyclin box. Hence, this cyclin box portion is believed to be a step necessary for binding to Cdk and controlling Cdk.
Furthermore, it is also recognized that the ability of cyclins to phosphorylate Cdk plays a critical role in the control of cell proliferation and through their ability cyclins bear close relation to phenomena such as cancer and immunity. Also, it is suggested that some cyclins are widely involved not only in the control of cell cycle, but also in the signal transmission.
Accordingly, there is strong likelihood that proteins having a high degree of homology in the amino acid sequence for the region called “cyclin box” as described above are cyclins. In this case, it is therefore anticipated that the proteins have the binding ability to Cdk and further that they have the ability to control kinase. See, Experimental Medicine, vol. 13, No. 6 (special issue), 1995.
DISCLOSURE OF INVENTION
The discovery and identification of novel protein having a high degree of homology to the amino acid sequence for the cyclin box which is characteristic of cyclins enables their use in elucidation of the detailed control mechanism of Cdk by cyclins as well as in the control of cell proliferation among others on the basis of the finding thus obtained. Further, it is thought that the elucidation of such protein with regard to variation of its quantity, its localization, its activation or the like within the cells brings knowledge useful to develop effective methods for the treatment of cancer or immune disorders, therapeutic agents therefor, methods for its diagnosis, diagnostic agents therefor, etc.
It is one object of this invention to discover and identify a novel protein having a high degree of homology to the amino acid sequence for the cyclin box which is characteristic of cyclins. A further object of the invention is to determine the amino acid sequence of the protein and to characterize a gene encoding the protein.
Also, it is an object of the invention to provide an expression vector into which an aforementioned genes is incorporated, a transformant into which the expression vector is introduced, and a recombinant protein obtained by growing the transformants.
Also, it is an object of the invention to provide a novel neuron marker based on the protein.
Further, it is another object of the invention to provide a method for screening cancer cells using the gene.
REFERENCES:
patent: 5247070 (1993-09-01), Yamada et al.
patent: 5798257 (1998-08-01), Zain et al.
patent: 96/02905 (1996-10-01), None
patent: 94/09135 (1994-04-01), None
Tony Hunter et al., “Cyclins and Cancer II: Cyclin D and CDK Inhibitors Come of Age”, The Salk Institute, Cell, vol. 79, pp. 573-582, Nov. 18, 1994.
Tamura et al., “Cyclin G: a new Mammalian Cyclin with Homology to Fission Yeast Cig1”, Research Institute for Microbial Diseases, Oncogene (1993), 8, pp. 2113-2118.
Hiroshi Matsushime, “Interaction Between Cyclins and cdks”, Experimental Medicine, vol. 13, No. 6 (special number) 1995, pp. 1-2.
Masahiko Ohtsuki, “Signal Transfer Factor in Focus (Intranuclear Factor Highlight 10)”, Experimental Medicine, vol. 12, No. 16, (Nov.) 1994, pp. 1-2.
Nakamura T. et al. “Cyclin 1: A New Cyclin Encoded by a Gene Isolated from Human Brain” Exp. Cell. Res. (Dec. 1995) vol. 221, No. 2, pp. 534-542.
Robert P. F. et al. “A Novel Cyclin Associates with M015/CDK7 to Form the CDK-Activating Kinase” Cell (1994) vol. 78, pp. 713-724.
Jonathon P. et al. “Isolation of a Human Cyclin cDNA: Evidence for Cyclin mRNA and Protein Regulation in the Cell Cycle and for Interaction with p34cdc2” Cell (1989), vol. 58, pp. 833-846.
Tony Hunter et al., “Cyclins and Cancer”, The Salk Institute, Cell, vol. 66, pp. 1071-1074, Sep. 20, 1991.
Daniel J. Lew et al., “Isolation of Three Novel Human Cyclins by Rescue of G1 Cyclin (Cln) Function in Yeast”, The Scripps Research Institute, Cell, vol. 66, pp. 1197-1206, Sep. 20, 1991.
John Lew et al., “Neuronal cdc2-like Kinase”, TIBS 20, Reviews, Elsevier Science Ltd. pp. 33-37, Jan. 1995.
Pillsbury Madison & Sutro LLP
Sumitomo Electric Industries Ltd.
Whisenant Ethan
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