Human conjunctival epithelial cell lines with an extended life s

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435467, 435371, C12Q 168

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060307897

ABSTRACT:
The present invention provides two human conjunctival epithelial cell lines with an extended life span, designated HC0597 and HC0708, and methods for producing such cell lines. These conjunctival cell lines can be cultured as stratified 3-dimensional (3-D) cultures. In turn, the 3-D cultures can be used as tissue- and species-specific cellular models of the human conjunctival ocular surface. These human conjunctival cultures are particularly useful, for example, in product safety evaluations to test products for their eye irritation potential.

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