Human chorionic gonadotropin beta-subunit nicking enzyme

Details Human chorionic gonadotropin beta-subunit nicking enzyme Human chorionic gonadotropin beta-subunit nicking enzyme

1994-08-31

1997-10-07

Weber, Jon P.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435212, C12N 950, C12N 948

Patent

active

056747276

ABSTRACT:
A substantially pure human chorionic gonadotropin beta subunit nicking enzyme (GBNE) has been purified from human blood serum. GBNE nicks the beta-subunit of human chorioinic gonadotropin at either Arg-44 or Gly-47, is an arginine specific metalloprotease, has an apparent molecular weight of between 150,000 and 430,000 daltons, and is partially inhibited by phenanthroline or leupeptin.

REFERENCES:
patent: 5252489 (1993-10-01), Macri
Nishimura et al. (1988) Endocrinology, 123(1), "Fragmentation of the .beta.-Subunit of Human Chorionic Gonadotropin Produced by Choriocarcinoma", pp. 420-425.
Bidart et al. (1988) Biochem. Biophys. Res. Comm., 154(2), "Characterization of a Cleavage Product in the Human Choriogonadotropin .beta.-Subunit", pp. 626-632.
Puisieux et al. (1990) Endocrinology, 126(2), "Occurrence of Fragmentation of Free and Combined Forms of the .beta.-Subunit of Human Chorionic Gonadotropin", pp. 687-694.
Birken et al. (1991) Endocrinology, 129(3), "The Heterogeneity of Human Chorionic Gonadotropin (hCG). II. Characteristics and Origins of Nicks in hCG Reference Standards", pp. 1551-1558.
Kardana et al. (1991) Endocrinology, 129(3), "The Heterogeneity of Human Chorionic Gonadotropin (hCG). I. Characterization of Peptide Heterogeneity in 13 Individual Preparations of hCG", pp. 1541-1550.
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Cole et al. (1991b) Endocrinology, 129(3), "The Heterogeneity of Human Chorionic Gonadotropin (hCG). III. The Occurrence and Biological and Immunological Activities of Nicked hCG", pp. 1559-1567.
Kardana et al. (1992) Clin. Chem., 38(1), "Polypeptide Nicks Cause Erroneous Results in Assays of Human Chorionic Gonadotropin Free .beta.-Subunit", pp. 26-33.
Kardana et al. (1994) J. Clin., Endocrin. Metab., 79(3), "Human Chorionic Gonadotropin .beta.-subunit Nicking Enzymes in Pregnancy and Cancer Patient Serum", pp. 761-767.
Kagimoto et al. (1995) Biol. Pharm. Bull., 18(6), "Nicked Human Chorionic Gonadotropin (hCG) by a Thermolytic Endoprotease", pp. 810-817, in Scisearch 95:450780.
Kardana et al. (1996) Oncol. Res., 8(1), "Gonadotropin .beta.-Subunit Nicking Enzyme (GBNE), A Potential Marker of Early Malignancies", pp. 13-16, in BIOSIS 96:229699.

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