Human centromere antigen polypeptide

Details Human centromere antigen polypeptide Human centromere antigen polypeptide

1992-05-07

1994-03-22

Nucker, Christine M.

Chemistry: analytical and immunological testing

For preexisting immune complex or auto-immune disease

Immune complex

436506, 530300, 435 71, G01N 33564

Patent

active

052963837

ABSTRACT:
The present invention provides polypeptides composing epitopes of human centromere protein B, genes encoding therefor, plasmids or phages containing the genes, transformants obtained by introducing the plasmids or phages containing the genes, a method for producing the human centromere antigen polypeptide using the transformant, and a method for detecting anti-centromere antibody using the human centromere antigen polypeptide. Analysis of the above-mentioned epitope was accomplished using CENP-B gene obtained from a cDNA library prepared using mRNAs isolated from Jurkat cells. The present invention allows the production of the human centromere protein B epitope region in a large quantity, which in turn allows the detection of human anti-centromere antibody readily and precisely using the peptide obtained. Furthermore, it becomes possible to make a precise classification of the disease type of a patient having human anti-centromere antibody by determinations using each of the epitopes.

REFERENCES:
Earnshaw et al: Analysis of anticentromere . . . CENP-B PNAS vol. 84 pp. 4979-4983 Jul. 1987.
Sullivan, K. F. et al., "CENP-B is a highly conserved mammalian centromere protein with homology to the helix-loop-helix family of proteins" Chromosoma (1991) 100:360-370.
Earnshaw et al., J. Cell Biol. (1987) 104:817-829.
Earnshaw et al., Proc. Natl. Acad. Sci. (1987) 84:4979-4983.
Rothfield et al., Arthritis and Rheumatism (1987) 30(12):1416-1419.

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