Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-05-11
1999-02-16
Elliott, George C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
4353201, 536 231, 536 235, C12Q 168, C12N 1512, C12N 1563
Patent
active
058719091
ABSTRACT:
The sequences encoding two isoforms of human cellular retinoid acid binding proteins, CRABP-I and CRABP-II, have been cloned and sequenced and are set forth with their corresponding amino acid sequences. The identification of human CRABP nucleic and amino acid sequences provides the basis for the construction of recombinant human CRABP vectors and expression constructs. Human CRABP can also be synthesized or produced ex vivo, e.g. in bacterial or other production systems. Ligand binding assays, including recombinant and chimeric receptor reporter assays, and direct and competition hybridization assays employing the human CRABP sequences herein described can be used to test drugs for retinoid induction and tissue specificity for pathologies in which retinoids are implicated. Immunoassays utilizing antibodies or binding fragments produced to human CRABP can also be used to test patient tissues for the presence and levels of CRABP for diagnosis and to monitor treatment. The identification of the nucleic and amino acids sequences for human CRABP-I and CRABP-II also contributes to the elucidation of the function and interaction of the retinoid-binding proteins.
REFERENCES:
patent: 4981784 (1991-01-01), Evans et al.
Sundelin, J. et al., "The Primary Structure of Rat Liver Cellular Retinol-binding Protein," J. Biol. Chem. 260:6488-6493 (1985).
Takase, S. et al., "Transfer of Retinoic Acid from Its Complex with Cellular Retinoic Acid-Binding Protein to the Nucleus," Arch. Biochem. Biophys. 247:328-334 (1986).
Thompson, C.B. et al., "Levels of c-myc Oncogene mRNA are Invariant Throughout the Cell Cycle," Nature 314:363-366 (1985).
Umesono, K et al., "Direct Repeats as Selective Response Elements for the Thyroid Hormone, Retinoic Acid, and Vitamin D.sub.3 Receptors," Cell 65:1255-1266 (1991).
Voorhees, J.J. et al., "Decreased Cyclic AMP in the Epidermis of Lesions of Psoriasis," Arch. Dermatol. 105:695-701 (1972).
Zelent, A. et al., "Cloning of Murine .alpha. and .beta. Retinoic Acid Receptors and a Novel Receptor .gamma. Predominantly Expressed in Skin," Nature 339:714-717 (1989).
Antras, J., et al., "Adipsin Gene Expression in 3T3-F442A Adipocytes Is Posttranscriptionally Down-Regulated by Retinoic Acid," J. Biol. Chem. 266:1157-1161 (1991).
Asselineau, D., et al., "Retinoic Acid Improves Epidermal Morphogenesis," Devel. Biol. 133:322-335 (1989).
Astrom, A., et al., "Cloning of CRABPII cDNA from Human Skin: Retinoic Acid Induces Expression of CRABPII but Not CRABPI in Human Skin in Vivo and in Dermal but Not Lung Fibroblasts in Vitro," J. Invest. Dermatol. 96:547 (1991).
Astrom, A., et al., "Retinoic Acid and Synthetic Analogs Differentially Activate Retinoic Acid Receptor Dependent Transcription," Biochem. Biophys. Res. Comm. 173:339-345 (1990).
Astrom, A., et al., "Molecular Cloning of Two Human Cellular Retinoic Acid-binding proteins (CRABP)," J. Biol. Chem. 266:17662-17666 (1991).
Astrom, A., et al., "Structure of the Human Cellular Retinoic Acid-Binding Protein II (CRABP-II) Gene: Early Transcriptional Regulation by Retinoic Acid," J. Biol. Chem. 267:25251-25255 (1992).
Bailey, J.S. et al., "Purification and Partial Characterization of a Novel Binding Protein for Retinoic Acid from Neonatal Rat," J. Biol. Chem. 263:9326-9332 (1988).
Benbrook, D., et al., "A New Retinoic Acid Receptor Identified from a Hepatocellular Carcinoma," Nature 333:669-672 (1988).
Berghard, A, et al., "Serum and Extracellular Calcium Modulate Induction of Cytochrome P 4501A1 in Human Keratinocytes," J. biol. Chem. 265:21086-21090 (1990).
Bossche, H. et al., "Cytochrome-P-450-Dependent Metabolism of Retinoic Acid in Rat Skin Microsomes: Inhibition by Ketoconazole," Skin Pharmaco. 1:176-185 (1988).
Boyce, S.T. et al., "Chapter 13: Normal Human Epidermal Keratinocytes,," in In Vitro Models for Cancer Research (Weber, M.M., and Sekely, L.I., eds) vol. 3, pp. 245-274. CRC Press, Boca Raton, FL (1986).
Brand, N. et al., "Identification of a Second Human Retinoic Receptor," Nature 332:850-853 (1988).
Busch, C. et al., "Tissue Distribution of Cellular Retinol-Binding Protein and Cellular Retinoic Acid-Binding Protein: Use of Monospecific Antibodies for Immunohistochemistry and cRNA for in Situ Localization of mRNA," Meth Enzymol. 189:315-324 (1990).
Cleveland, D.W. et al., "Multiple Determinants of Eukaryotic mRNA Stability," New. Biol. 1:121-126 (1989).
Danielson, P.E. et al., "p1B15: A cDNA Clone of the Rat mRNA Encoding Cyclophilin," DNA 7:261-267 (1988).
Darmon, M. et al., "Biological Activity of Retinoids Correlates with Affinity for Nuclear Receptors but Not for Cytosolic Binding Protein," Skin Pharmacol. 1:161-175 (1988).
DeThe, H. et al., "Differential expression and ligand regulation of the retinoic acid receptor .alpha. and .beta. genes," EMBO J. 8:429-433 (1989).
DeThe, H. et al., "Identification of a Retinoic Acid Rsponsive Element in the Retinoic Acid Receptor .beta. Gene," Nature 343:177-180 (1990).
Edwards, S.A. et al., "The Transcription Factor Egr-1 Is Rapidly Modulated in Response to Retinoic Acid in P19 Embryonal Carcinoma Cells," Dev. Biol. 148:165-173 (1991).
Eichele, G., "Retinoids and Vertebrate Limb Pattern Formation," Trends Genet. 5:246-251 (1989).
Elder, J.T. et al., "Retinoic Acid Receptor Gene Expression in Human Skin," J. Invest. Dermatol. 96:425-433 (1991).
Elder, J.T. et al., "Protooncogene Expression in Normal and Psoriatic Skin," J. Invest Dermatol. 94:19-25 (1990).
Feinberg, A.P. et al., "A Technique for Radiolabellig DNA Restriction Endonuclease Fragments to High Specific Activity," Anal. Biochem. 132:6-13 (1983).
Fiorella, P.D. et al., "Expression of Cellular Retinoic Acid Binding Protein (CRABP) in Escherichia coli, " J. Biol. Chem. 266:16572-16579 (1991).
Floyd, E.E. et al., "Regulation of Type I (Epidermal) Transglutaminase mRNA Levels during Squamous Differentiation: Down Regulation by Retinoids," Mol. Cell Biol. 9:4846-4851 (1989).
Gassman, M. et al., "Efficient Production of Chicken Egg Yolk Antibodies Against a Conserved Mamalian Protein," FASEB 4:2528-2532 (1990).
Giguere, V. et al., "Identification of a Receptor for the Morphogen Retinoic Acid," Nature 330:624-629 (1987).
Glass, C.K et al., "Positive and Negative Regulation of Gene Transcription by a Retinoic Acid-Thyroid Hormone Receptor Heterodimer," Cell 59:697-708 (1989).
Greenberg, M.E. et al., "Stimulation of 3T3 Cells Induces Transcription of the C-fos Proto-Ocogene," Nature 311:433-438 (1984).
Harper, R.A. et al., "Human Skin Fibroblasts Derived from Papillary and Reticular Dermis: Differences in Growth Potential in vitro," Science 204:526-527 (1979).
Heyman, R.A. et al., "9-Cis Retinoic Acid Is a High Affinity Ligand for the Retinoid X Receptor," Cell 68:397-406 (1992).
Hirschel-Scholz, S. et al., "Ligand-Specific and Non-Specific In Vivo Modulation of Human Epidermal Cellular Retinoic Acid Binding Protein (CRABP)," Eur. J. Clin. Invest. 19:220-227 (1989).
Kadonaga, J.T. et al., "Promoter-Specific Activation of RNA Polymerase II Transcription by Sp1," Trends Biochem. Sci. 11:20-23 (1986).
Kitamoto, J. et al., "The Presence of a Novel Cellular Retinoic Acid-Binding Protein in Chick Embryos: Purification an partial Characterization," Biochem. Biophys. Res. Comm. 157:1302-1308 (1988).
Kopan, R. et al., "The Use of Retinoic Acid to Probe the Relation Between Hyperproliferation-Associated Keratins and Cell Proliferation in Normal and Milignant Epidermal Cells," J. Cell Biol. 109:295-307 (1989).
Krust, A. et al., "A Third Human Retinoic Acid Receptor, hRAR-.gamma.," PNAS (USA) 86:5310-5314 (1989).
Leask, A. et al., "Transcription Factor AP2 and its Role in Epidermal-Specific Gene Expression," PNAS (USA) 88:7948-7952 (1991).
Leid, M. et al., "Purification, Cloning, and RXR Identity of the HeLa Cell Factor with Which RAR or TR Heterodimerizes to Bind Target Sequences Efficiently," Cell 68:377-395 (1992).
Lemaire, P. et al., "the Serum-Inducible Mouse Gene Krox-24 Encodes a Sequence-Specific Transcriptional Activator," Mol. Cell. Biol. 10:3456-3467 (1990).
Levin, A.A. et al., "9-Cis Retinoic Acid Stereoisomer Binds an
.ANG.strom Anders
Pettersson Ulrika
Tavakkol Amir
Voorhees John J.
Elliott George C.
McKelvey Terry A.
The Regents of the University of Michigan
LandOfFree
Human cellular retinoic acid binding protein I and II DNA and me does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Human cellular retinoic acid binding protein I and II DNA and me, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Human cellular retinoic acid binding protein I and II DNA and me will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2060991