Human cell line and triomas, antibodies, and transformants deriv

Details Human cell line and triomas, antibodies, and transformants deriv Human cell line and triomas, antibodies, and transformants deriv

1987-05-27

1991-03-19

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524027, 4351722, 435948, 935 89, 935 95, 935 99, 935100, 935 93, C12N 524, C12N 1507

Patent

active

050010654

ABSTRACT:
A stable, continuous human cell line or progeny thereof is produced that is resistant to 6-thioguanine and ouabain, secretes less than 40 ng/ml of endogenous IgM antibodies, and grows with a doubling time of about 18 hours. The cell line, which preferably is adapted to serum-free medium, may be used as a fusion partner with an antibody-producing cell line so as to generate antibodies. In addition, it may be electroporated with a vector containing a gene of interest to produce a transformed cell line which generates a protein encoded by the gene, such as an IgG or IgM antibody.

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Kozbor et al., Proc. Natl. Acad. Sci. U.S.A., 79:6651-6655 (1982).
Kozbor et al., Human Hybridomas and Monoclonal Antibodies, Engleman et al., eds. (Plenum Press, 1985) pp. 21-36.
O'Hare et al., Protides of the Biological Fluids, Proceedings of the 13th Colloquium, 1982, H. Peeters, ed. (Oxford: Pergamon Press, 1983) pp. 265-268.
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Oestberg et al., Hybridoma, 2(4), 361-367 (1983).

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