Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-05-09
1999-11-30
Carlson, Karen Cochrane
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 231, 435197, C12Q 168, C07H 1700, C12N 918
Patent
active
059940743
ABSTRACT:
Two previously undescribed human cdc25 genes, designated cdc25 A and cdc25 B, which have been shown to have an endogenous tyrosine phosphatase activity that can be specifically activated by B-type cyclin, in the complete absence of cdc2 are described. As a result of this work, new approaches to regulating the cell cycle in eukaryotic cells and, particularly, to regulating the activity of tyrosine specific phosphatases which play a key role in the cell cycle are available. Applicant's invention relates to methods of regulating the cell cycle and, specifically, to regulating activation of cdc2-kinase, through alteration of the activity and/or levels of tyrosine phosphatases or through alteration of the interaction of components of MPF. The present invention also relates to agents or compositions useful in the method of regulating (inhibiting or enhancing) the cell cycle. Such agents or compositions can be inhibitors (such as low molecular weight peptides or compounds, either organic or inorganic) of the catalytic activity of tyrosine specific PTPases (particularly cdc25), blocking agents which interfere with interaction or binding of the tyrosine specific PTPase with cyclin or the cyclin/cdc2 complex, or agents which interfere directly with the catalytic activity of the PTPases. The invention also pertains to an assay for identifying agents which after stimulation of kinase activity of pre-MPF and thus alter activation of MPF and entry into mitosis. Such agents are also the subject of this invention.
REFERENCES:
patent: 5262409 (1993-11-01), Margolis et al.
patent: 5294538 (1994-03-01), Beach
patent: 5441880 (1995-08-01), Beach et al.
patent: 5695950 (1997-12-01), Beach et al.
Barratte et al., "Screening for Antimitotic Compounds Using the cdc25 Tyrosine Phosphatase, an Activator of the Mitosis-inducing p34cdc2/cyclinBcdc13 Protein Kinase", Anticancer Research 12: 873-880 (1992).
Camonis et al., "Characterization, Cloning and Sequence Analysis of the CDC25 Gene which Controls the Cyclic AMP Level ofSaccharomyces cerevisiae", Embo J 5: 375-380 (1986).
Daniel et al., "Clones from Two Different Genomic Regions Complement the cdc25 Start Mutation of Saccharomyces cerevisiae", Curr Genet. 10: 643-646 (1986).
Daniel, "The cdc25 Start Gene of Saccharomyces cerevisiae: Sequencing of the Active C-terminal Fragment and Regional Homologies with Rhodopsin and Cytochrome P450", Curr. Genet 10: 879-885 (1986).
Dunphy et al.. "The cdc25 Protein Contains an Intrinsic Phosphatase Activity", Cel 1 67: 189-196 (1991).
Galaktionov et al., "Specific Activation of cdc25 Tyrosine Phosphatases by B-type Cyclins: Evidence for Multiple Roles of Mitotic Cyclins", Cell 67: 1181-1194 (1991).
Gautier et al., "Cdc25 is a Specific Tyrosine Phosphatase the Directly Activates p34cdc2", Cell 67: 197-211 (1991).
Gould et al., "Complementation of the Mitotic Activator, p80cdc25, by a Human Protein-Tyrosine Phosphatase", Science 250: 1573-1576 (1990).
Jessus et al., "Oscillation of MPF is Accompanies by Periodic Association between cdc25 and cdc2-Cyclin B", Cell 68: 323-332 (1982).
Jimenez et al., "Complementation of Fission Yeast cdc2ts and cdc25ts Mutants Identifies Two Cell Cycle Genes from Drosophila: a cdc25 Homologue and String", Embo J 9: 3565-3571 (1990).
Kakizuka et al., "A Mouse cdc25 Homolog is Differentially and Developmentally Expressed", Genes & Development 6: 578-590 (1992).
Kumagai et al., "The cdc25 Protein Controls Tyrosine Dephosphorylation of the cdc25 Protein in a Cell-Free System", Cell 64: 903-914 (1991).
Lee et al., "cdc25+ Encodes a Protein Phosphatase that Dephosphorylates p34cdc2". Mol. Biol. 3: 73-84 (1992).
Lerner, R.A. "Tapping the Immunological Repertoire to Produce Antibodies of Predetermined Specificity", Nature 299: 592-296 (1982).
Millar et al., "p55cdc25 is a Nuclear Protein Required for the Initiation of Mitosis in Human Cells", Proc. Natl. Acad. Sci. USA 88: 10500-10504 (1991).
Millar et al., "p80cdc25 Mitotic Inducer is the Tyrosine Phosphatase that Activates p34cdc2 Kinase in Fission Yeast", Embo J 10: 4301-4309 (1991).
Millar et al., "The cdc25 M-phase Inducer: An Unconventional Protein Phosphatase" Cell 68: 407-410 (1992).
Moreno et al., "Clues to Action of cdc25 Protein", Nature 351: 194 (1991).
Nagata et al., "An Additional Homolog of the Fission Yeast cdc25+ Gene Occurs in Humans and is Highly Expressed in Some Cancer Cells" The New Biologist 3: 959-968 (1991).
Ogden et al., "Isolation of a Novel Type of Mutation in the Mitotic Control of Schizosaccaromyces Pombe whose Phenotypic Expression is Dependent on the Genetic Background and Nutritional Environment", Curr Genet. 10: 509-514 (1986).
Ohno et al., "A yeast Gene Coding for a Putative Protein Kinase Homologous to cdc25 Suppressing Protein Kinase", FEBS 222: 279-285 (1987).
Osmani et al., "Parallel Activation of the NIMA and p34cdc2 Cell Cycle-Regulated Protein Kinases is Required to Initate Mitosis in A. Nidulans", Cell 67: 283-291 (1991).
Russell et al., "cdc25+ Functions as an Inducer in the Mitotic Control of Fission Yeast", Cell 45: 145-153 (1986).
Sadhu et al., "Human Homolog of Fission Yeast cdc25 Mitotic Inducer is Predominantly Expressed in G2", Proc. Natl. Acad. Sci. USA 87: 5139-5143 (1990).
Strausfeld et al., "Dephosphorylation and Activation of a p34cdc2/Cyclin B Complex In Vitro by Human CDC25 Protein", Nature 351: 242-245 (1991).
Beach David H.
Galaktionov Konstantin
Carlson Karen Cochrane
Cold Spring Harbor Laboratories
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