Human .beta.1-6 n-acetylglucosaminyl transferase

Details Human .beta.1-6 n-acetylglucosaminyl transferase Human .beta.1-6 n-acetylglucosaminyl transferase

1992-10-01

1994-11-01

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435697, 435 97, C12N 910, C12P 1918

Patent

active

053607333

ABSTRACT:
The present invention provides a novel .beta.1.fwdarw.6 N-acetylglucosaminyltransferase, which forms core 2 oligosaccharide structures in O-glycans, and a novel acceptor molecule, leukosialin, CD43, for core 2 .beta.1.fwdarw.6 N-acetylglucosaminyltransferase activity. The amino acid sequences and nucleic acid sequences encoding these molecules, as well as active fragments thereof, also are disclosed. A method for isolating nucleic acid sequences encoding proteins having enzymatic activity is disclosed, using CHO cells that support replication of plasmid vectors having a polyoma virus origin of replication. A method to obtain a suitable cell line that expresses an acceptor molecule also is disclosed.

REFERENCES:
Bierhuizen, M. F. A. and Fukuda, M.; Proc. Natl. Acad. Sci. U.S.A. 89:9326-9330 (1992).
Ropp, P. A. et al.; J. Biol. Chem. 266:23863-23871 (1991).
Saitoh, O. et al.; Blood 77:1491-1499 (1991).
Toone, E. J. et al.; Tetrahedron 45:5365-5422 (1989).
Fukuda, M. et al., "Structures of O-linked Oligosaccharides Isolated from Normal Granulocytes, Chronic Myelogenous Leukemia Cells and Acute Myelogenous Leukemia Cells," J. Biol. Chem. 261:12796-12806 (1986).
Heffernan, M., and Dennis, J. W., "Polyoma and Hamster Papovavirus Large T Antigen-Mediated Replication of Expression Shuttle Vectors in Chinese Hamster Ovary Cells," Nucl. Acids Res. 19:85-92 (1991).
Kuwoska-Latallo, J. F. et al., "A Cloned Human cDNA Determines Expression of a Mouse Stage-Specific Embryonic Antigen and the Lewis Blood Group .alpha.(1,3/1,4) Fucosyltransferase," Genes & Dev. 4:1288-1303 (1990).
Lowe, John B. et al., "ELAM-1-Dependent Cell Adhesion to Vascular Endothelium Determined by a Transfected Human Fucosyltransferase cDNA," Cell. 63:475-484 (1990).
Piller, F., et al., "Altered O-glycan Synthesis in Lymphocytes from Patients with Wiskott-Aldrich Syndrome," J. Exp. Med. 173:1501-1510 (1991).
Schachter, H. et al., "Enzymes Associated with Glycosylation," Current Opinion on Structural Biology 1:755-765 (1991).

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