Human Bek Fibroblast growth factor receptor

Details Human Bek Fibroblast growth factor receptor Human Bek Fibroblast growth factor receptor

1995-05-26

1999-01-26

Teng, Sally P.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

530350, 530395, 530399, 435691, C07K 14705

Patent

active

058638881

ABSTRACT:
The complete cDNA cloning of two human genes previously designated flg and bek is disclosed. These genes encode for two similar but distinct surface receptors comprised of an extracellular domain with three immunoglobulin-like regions, a single transmembrane domain, and a cytoplasmic portion containing a tyrosine kinase domain with a typical kinase insert. The expression of these two cDNAs in transfected NIH-3T3 cells led to the biosynthesis of proteins of 150 kDa and 135 kDa for flg and bek respectively. Direct binding experiments with radiolabeled acidic FGF (aFGF), basic FGF (bFGF), or kFGF inhibition of binding with native growth factors, and Scatchard analysis of the binding data indicated that bek and flg bind aFGF, bFGF, or kFGF with dissociation constants of (2-15).times.10.sup.-11 M. The high affinity binding of three distinct growth factors to each of two different receptors represents a unique double redundancy without precedence among polypeptide growth factor/receptor interactions. The use of transformed host cells overexpressing fig or bek or biologically active fragments thereof for drug screening is disclosed.

REFERENCES:
J. Biol. Chem. 260: 13860-13868, 1985, Neufeld Gospodarowicz, English Original.
J. Biol. Chem. 263: 14023-14029, 1988, Feige Baird English Original.
Biochem. Biophys. Res. Commun. 155: 583-590, 1988, Imamura Tokita Mitsui, English Original.
Molec. and Cell. Biol. 8: 5541-5544, 1988, Kornbluth Paulson Hanafusa, English Original.
Oncogene 3: 9-15, 1988, Ruta Howk Ricca, Drohan Zabelshansky et al., English Original.
J. Biol. Chem. 264: 18647-18653, 1989, Burrus Olwin, English Original.
Science 245: 57-60, 1989, Lee Johnson Cousens, Fried Williams, English Original.
Proc. Natl. Acad. Sci. USA 86: 5449-5453, 1989, Pasquale singer, English Original.
Proc. Natl. Acad. Sci. USA 86: 8722-8726, 1989, Ruta Burgess Givol, Epstein Neiger et al., English Original.
Nucleic Acids Res. 18: 1906, 1990, Isacchi Bergonzoni Sarmientos, English Original.
Science 248: 1410-1413, 1990, Kaner Baird Mansukhani, Basilico Summers et al., English Original.
Proc. Natl. Acad. Sci. USA 87: 1596-1600, 1990, Reid Wilks Bernard, English Original.
Cold Spring Harbor Laboratory, 1982, Maniatis et al., English Original.
Green Publishing Assoc. and Wiley Interscience, 1987, Ausubel, et al., eds., English Original.
Biochem. Biophys. Res. Commun. 169: 680-685, 1990, Itoh Terachi Ohta, Seo, English Original.
Molec. Cell. Biol. 11:4627-4634, Sep. 1991, Johnson Lu Chen, Werner Williams, English Original.
Growth Factors 5:115-127, 1991, Kiefer Baird Nguyen, George-Nascime Mason et al., English Original.
Molec. Cell. Biol. 11:5068-5078, 1993, Mohammadi Honegger Rotin, Fischer Bellot et al., English Original.
WO 91/00916, Jul. 6, 1990, Jan. 24, 1991, Williams Johnson Lee, English Original.
Sambrook et al., Molecular Cloning, A lab. Manual, Second Edition, vol. 3, pp. 16.2-16.30 and 71.2-17.28, 1989 Cold Spring Harbor Lab. Press, Cold Spring Harbor.
Duan et al., J. of Biol. Chem., 266, 413-418, 1991.

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