Human anti-RH(D) monoclonal antibodies, cell lines and methods o

Drug – bio-affecting and body treating compositions – Immunoglobulin – antiserum – antibody – or antibody fragment,... – Monoclonal antibody or fragment thereof

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4241531, 53038815, 5303887, 435 725, 43524027, 4351722, 435 7021, A61K 39395, C07K 1634, C12N 522, G01N 3353

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054965489

ABSTRACT:
The present invention provides human monoclonal antibodies having the following essential characteristics: (a) binding to Rh(D) antigen, but not C, c, E or e antigens of the Rh blood group system; (b) being IgG3 proteins; (c) having kappa light chains; (d) being of the allotype G3m(21); (e) binding to D.sup.u cells by an indirect antiglobulin test; (f) binding to D.sup.IV, D.sup.V and D.sup.VII variant antigens; and (g) not binding to D.sup.VI or D.sup.B variant antigens, which may be employed for Rh-typing of red blood cells and passive immunization to prevent hemolytic disease of the newborn. Such a monoclonal antibody is exemplified by the monoclonal antibody of cell line ECACC 87091606 deposited at the European Collection of Animal Cell Cultures, Public Health Laboratory Service Center for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, SP4, OJG, England of Sep. 16, 1987.

REFERENCES:
Lomas et al., Transfusion 26:560, 1986.
Tippett, Medical Laboratory Sciences 45: 88-93, 1988.
Bron et al., PNAS USA 81:3214-3217 1984.
McCann et al. J. Immunological Mtds. 115(1):3-15 1988.
Thomson et al., The Lanlet 336:1147-1150, 1990.

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