Human and rat gamma glutamyl hydrolase genes

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 6, 435 691, 435195, 4353201, 435325, 435353, 435366, 435372, 536 231, 536 232, 536 235, 536 2431, C12N 1500, C12N 1585, C07H 2102, C12Q 168

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058010319

ABSTRACT:
The present invention is directed to isolated nucleic acid molecules encoding gamma glutamyl hydrolase (GH). Expression vectors and host cells comprising the nucleic acid molecules are also provided, as well as methods for increasing or decreasing the expression of GH in host cells. The invention further provides a method of screening a substance for the ability of the substance to modify GH function, and a method for isolating other GH molecules. DNA oligomers and antibodies specific for GH are provided, each of which can be used to detect GH in a sample. Methods for decreasing deleterious side effects of antifolate treatment, increasing the levels of GH in cells of a patient, increasing the effectiveness of antifolate treatment, and monitoring progression of a tumor are further provided.

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Chandler et al., "Functional Specificity of Jejunal Brush-Border Pteroylpolyglutamate Hydrolase in Pig," American J. of Physiology, 260:G865-G872 (1991).
Wang et al., "The Properties of the Secreted .gamma.-glutamyl Hydrolases from H35 Hepatoma Cells," Biochim. Biophys. Acta, 1164:227-235 (1993).
McGuire, J.J. and Coward, J.K., in "Folates and Pterins: vol. 1 Chemistry and Biochemistry of Folates" (Blakely, R.L. and Benkovic, eds.) John Wiley & Sons, Inc., New York, 135-190 (1984).
Acc. #T26504, N-Gene Seq, Database.
Hillier et al., Acc. Nos. N45706, R7701, N53343 H09442, & N48195 in E51 Database , Jun. 1995 to Feb. 1996.

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