Human and mouse uroplakin II gene transcriptional regulatory...

Chemistry: molecular biology and microbiology – Vector – per se

Reexamination Certificate

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Details

C435S235100, C435S006120, C435S069100, C435S069700, C435S455000, C514S04400A, C536S023100, C536S023400, C536S024100

Reexamination Certificate

active

06852528

ABSTRACT:
The invention provides new urothelial cell specific transcriptional regulatory sequences derived from human uroplakin II (hUPII), as well as polynucleotide constructs such as adenoviral vectors and methods of using hUPII-derived TREs. Additionally, the invention provides adenoviral vectors comprising a gene, preferably an adenovirus gene, under transcriptional control of a urothelial cell-specific transcriptional regulatory element (TRE). These vectors display urothelial cell-specific cytotoxicity, which is especially useful in the context of bladder cancer, in which destruction of these cells is desirable. The invention further provides compositions and host cells comprising the vectors, as well as method of using the adenoviral vectors.

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Lin, Jun-Hsiang et al., “A tissue-specific promoter that can drive a foreign gene to express in the suprabasal urothelial cells of transgenic mice,”Proc. Natnl. Acad. Sci. USA, vol. 92, pp. 679-683, Jan. 1995.

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