Human aflatoxin B1 aldehyde reductase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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C12N 904

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active

059812443

ABSTRACT:
The invention provides a human aflatoxin B1 aldehyde reductase (AFB1-hAR) and polynucleotides which identify and encode AFB1-hAR. The invention also provides expression vectors, host cells, agonists, antibodies and antagonists. The invention also provides methods for treating disorders associated with expression of AFB1-hAR.

REFERENCES:
patent: 5795761 (1998-08-01), Powers et al.
Hayes, J.D., et al., "Resistance to Aflatoxin B1 Is Associated with the Expression of a Novel Aldo-Keto Reductase Which Has Catalytic Activity Towards a Cytotoxic Aldehyde-containing Metabolite of the Toxin", Cancer Research, 53:3887-3894 (1993).
Judah, D.J., et al., "A novel aldehyde reductase with activity towards a metabolite of aflatoxin B1 is expressed in rat liver during carcinogenesis and following the administration of an anti-oxidant", Biochem J, 292:13-18 (1993).
Ellis, E.M., et al., "An ethoxyquin-inducible aldehyde reductase from rat liver that metabolizes aflatoxin B.sub.1 defines a subfamily of aldo-keto reductases", Proc Natl Acad, USA, 90:10350-10354 (1993).
McGlynn, K.A., et al., "Susceptibility to hepatocellular carcinoma is associated with genetic variation in the enzymatic detoxification of aflatoxin B.sub.1 ", Proc Natl Acad Sci, USA, 92:2384-2387 (1995).
Aguilar, F., et al., "Aflatoxin B.sub.1 induces the transversion of G.fwdarw. T in codon 249 of the p53 tumor suppressor gene in human hepatocytes", Proc Natl Acad Sci USA, 90:8586-8590 (1993).
Ellis, E.M., (GI 433611) GenBank Sequence Database (Accession X74673), National Center for Biotechnology Information: National Library of Medicine, Bethesda, Maryland 20849 No Date Provided.
Ellis, E.M., (GI 433610) GenBank Sequence Database (Accession X74673), National Center for Biotechnology Information: National Library of Medicine, Bethesda, Maryland 20819 No Date Provided.
Wang, A. et al., "Cloning, Expression, and Catalytic Mechanism of Murine Lysophospholipase I", J. Biol. Chem., May 9, 1997, 272(19) : 12723-12729.

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