Human aflatoxin B1 aldehyde reductase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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4352523, 43525233, 435325, 4353201, 435419, 43525411, 4352543, 536 232, 536 2431, C12N 904, C12N 1509, C12N 1570, C12N 1553

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059196858

ABSTRACT:
The invention provides a human aflatoxin B1 aldehyde reductase (AFB1-hAR) and polynucleotides which identify and encode AFB1-hAR. The invention also provides expression vectors, host cells, agonists, antibodies and antagonists. The invention also provides methods for treating disorders associated with expression of AFB1-hAR.

REFERENCES:
Hayes, J.D., et al., "Resistance to Aflatoxin B1 Is Associated with the Expression of a Novel Aldo-Keto Reductase Which Has Catalytic Activity Towards a Cytotoxic Aldehyde--containing Metabolite of the Toxin", Cancer Research, 53:3887-3894 (1993).
Judah, D.J., et al., "A novel aldehyde reductase with activity towards a metabolite of aflatoxin B1 is expressed in rat liver during carcinogenesis and following the administration of an anti-oxidant", Biochem J, 292:13-18 (1993).
Ellis, E.M., et al., "An ethoxyquin-inducible aldehyde reductase from rat liver that metabolizes aflatoxin B.sub.1 defines a subfamily of aldo-keto reductases", Proc. Natl Acad Sci, USA, 90:10350-10354 (1993). (GI 433610) (GI 433611).
McGlynn, K.A., et al., "Susceptibility to hepatocellular carcinoma is associated with genetic variation in the enzymatic detoxification of aflatoxin B.sub.1 ", Proc. Natl Acad Sci, USA, 92:2384-2387 (1995).
Aquilar, F., et al., "Aflatoxin B.sub.1 induces the transversion of G.fwdarw. T in codon 249 of the p53 tumor suppressor gene in human hepatocytes", Proc Natl Acad Sci USA, 90:8586-8590 (1993).
Ellis, E.M., (GI 433611) GenBank Sequence Database (Accession X74673), National Center for Biotechnology Information: National Library of Medicine, Bethesda, Maryland 20849 No Year Provided.
Ellis, E.M., (GI 433610) GenBank Sequence Database (Accession X74673), National Center for Biotechnology Information: National Library of Medicine, Bethesda, Maryland 20849 No Year Provided.
Ellis et al. "An ethoxyquin-inductible aldehyde reductase from rat liver that metabolizes aflatoxin B1 defines a subfamily of aldo-keto reductases" Proc. Natl. Acad. Sci. U.S.A. 90 (21), 10350-10354 (1993), Nov. 1, 1993.
Genbank Accession X74673 "R.norvegicus AFAR mRNA for aflatoxin B1 aldehyde reductase." Submitted by E.M. Ellis, May 9, 1996.
Genbank Accession Q60770 "Human brain Expressed Sequence Tag EST00883" Submitted by MD Adams et al, Mar. 16, 1994.
Wells et al. "Additivity of mutational effects in proteins" Biochemistry 29 (37), 8509-8517, Sep. 18, 1990.

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