Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Reexamination Certificate
2006-09-19
2006-09-19
Andres, Janet L. (Department: 1649)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Reexamination Certificate
active
07109296
ABSTRACT:
DNA encoding human 5-HT3-C has been cloned and characterized. The recombinant protein is capable of forming biologically active human 5-HT3-C protein. The cDNA has been expressed in recombinant host cells that produce active recombinant protein. In addition, the recombinant host cells are utilized to establish a method for identifying modulators of the receptor activity, and receptor modulators are identified.
REFERENCES:
patent: 6462188 (2002-10-01), Kirkness
patent: WO 0071741 (2000-11-01), None
patent: WO 0202639 (2002-01-01), None
Bruss M. et al. Molecular cloning of alternatively spliced human 5-HT3 receptor cDNAs, Ann. N.Y. Acad. Sci. 861: 234-235 (1998).
Barnard, “The Transmitter-Gated Channels: A Range Of Receptor Types And Structures,”Trends Pharmacol. Sci., vol. 17, pp. 305-309 (1996).
Belelli et al., “Cloning And Functional Expression Of A Human 5-Hydroxytryptamine Type-3AsReceptor Subunit,”Mol. Pharmacol., vol. 48, pp. 1054-1062 (1995).
Boess et al., “Analysis Of The Ligand Binding Site Of The 5-HT3Receptor Using Site Directed Mutagenesis: Importance Of Glutamate 106,”Neuropharm., vol. 36, pp. 637-647 (1997).
Bufton et al., “Distribution And Characterization Of The [3H]Granisetron-Labelled 5-HT3Receptor In The Human Forebrain,”Neuropharm., vol. 32(12), pp. 1325-1331 (1993).
Davies et al., “The 5-HT3-BSubunit Is A Major Determinant Of Serotonin-Receptor Function”,Nature(London), vol. 397, pp. 359-363 (1999).
Derkach et al., “5-HT3Receptors Are Membrane Ion Channels,”Nature(London), vol. 339, pp. 706-709 (1989).
Fletcher et al., “Desperately Seeking Subunits: Are Native 5-HT3Receptors Really Homomeric Complexes?”Trends Pharmacol. Sci., vol. 19, pp. 212-215 (1998).
Fletcher et al., “Purification Of 5-Hydroxytryptamine-3Receptors From Porcine Brain”,Br. J. Pharmacol., vol. 122, pp. 655-662 (1997).
Fletcher et al., “Evidence That Porcine Native 5-HT3Receptors Do Not Contain Nicotinic Acetylcholine Receptor Subunits”,Neuropharm., vol. 37, pp. 397-399 (1998).
Furutani et al., “Novel Mechanism Associated With An Inherited Cardiac Arrhythmia: Defective Protein Trafficking By The Mutant HERG (G601S) Potassium Channel”,Circulation, vol. 99, pp. 2290-2294 (1999).
Gralla, “Antiemetic Therapy,”Semin. Oncol., vol. 25, pp. 577-583 (1998).
Greenshaw et al., “The Non-Antiemetic Uses Of Serotonin 5-HT3Receptor Antagonists: Clinical Pharmacology and Therapeutic Applications,”Drugs, vol. 53(1), pp. 20-39 (1997).
Gurley et al., “Nicotinic Agonists Competitively Antagonize Serotonin At Mouse 5-HT3Receptors Expressed In Xenopus Oocytes,”Neurosci. Letters, vol. 247, pp. 107-110 (1998).
Hugnot et al., “Kv8.1, A New Neuronal Potassium Channel Subunit with Specific Inhibitory Properties TowardsShabAndShawChannels,”EMBO J., vol. 15(13), pp. 3322-3331 (1996).
Jan et al., “Voltage-Gated And Inwardly Rectifying Potassium Channels,”J. Physiology, vol. 505(2), pp. 267-282 (1997).
Lambert et al., “5-HT3 Receptors”In Ligand- Voltage-Gated Ion Channels, (R. North (Ed.), CRC Press—Boca Raton, Fl), Chp. 5, pp. 177-211 (1995).
Lummis et al., “Radioligand Binding And Photoaffinity Labeling Studies Show A Direct Interaction Of Phenothiazines At 5-HT3Receptors,”Neuropharm., vol. 36(4/5), pp. 665-670 (1997).
Lummis et al., “Solubilization, Purification, And Functional Reconstitution Of 5-Hydroxytryptamine3Receptors From N1E-115 Neuroblastoma Cells,”Mol. Pharmacol., vol. 41, pp. 18-23 (1992).
Lummis et al., “Characterization Of [3H]Meta-chlorophenylbiguanide Binding To 5-HT3Receptors In N1E-115 Neuroblastoma Cells,”Eur. J. Pharmacol., vol. 243, pp. 7-11 (1993).
Maricq et al., “Primary Structure And Functional Expression Of The 5HT3Receptor: A Serotonin-Gated Ion Channel,”Science(Washington, D. C.), vol. 254, pp. 432-437 (1991).
Mathur et al., “Ile-177 And Ser-180 In The S1 Segment Are Critically Important In Kv1.1 Channel Function,”J. Biol. Chem., vol. 274(17), pp. 11487-11493 (1999).
Mikayama et al., “Molecular Cloning And Functional Expression Of A cDNA Encoding Glycosylation-Inhibiting Factor,”Proc. Natl. Acad. Sci. USA, vol. 90, pp. 10056-10060 (1993).
Miller et al., “Membrane-Bound And Solubilized Brain 5HT3Receptors: Improved Radioligand Binding Assays Using Bovine Area Postrema Or Rat Cortex And The Radioligands3H-GR65630,3H-BRL43694, And3H-LY278584,”Synapse(NY), vol. 11, pp. 58-66 (1992).
Miyake et al., “Molecular Cloning of Human 5-Hydroxytryptamine3Receptor: Heterogeneity In Distribution And Function Among Species,”Mol. Pharmacol., vol. 48, pp. 407-416 (1995).
Passani et al., “Therapeutic Potentials of Itasetron (DAU 6215), A Novel 5-HT3Receptor Antagonist, In The Treatment Of Central Nervous System Disorders,”CNS Drug Rev., vol. 2, pp. 195-213 (1996).
Peters et al., “Recent Advances in the Electrophysiological Characterization of 5-HT3Receptors,”Trends Pharmacol. Sci., vol. 13, pp. 391-397 (1992).
Salinas et al., “Modes of Regulation OfShabK+Channel Activity By The Kv8.1 Subunit,”J. Biol. Chem., vol. 272(13), pp. 8774-8780 (1997).
Salinas et al., “New Modulatory α Subunits For MammalianShabK+Channels,”J. Biol. Chem., vol. 272(39), pp. 24371-24379 (1997).
Shalaby et al., “Dominant-NegativeKvLQT1Mutations Underlie The LQT1 Form Of Long QT Syndrome,”Circulation, vol. 96(6), pp. 1733-1736 (1997).
Shuck et al., “Cloning And Characterization Of Two K+Inward Rectifier (Kir) 1.1 Potassium Channel Homologs From Human Kidney (Kir1.2 and Kir1.3),”J. Biological Chem., vol. 272(1), pp. 586-593 (1997).
Steward et al., “Labeling of 5-HT3Receptor Recognition Sites In The Rat Brain Using The Agonist Radioligand [3H]Meta-chlorophenylbiguanide,”Eur. J. Pharmacol., vol. 243, pp. 13-18 (1993).
Stocker et al., “Subunit Assembly And Domain Analysis Of Electrically Silent K+Channel (alpha)-Subunits Of The Rat Kv9 Subfamily,”J. Neurochem., vol. 72(4), pp. 1725-1734 (1999).
Sugita et al., “5-Hydroxytryptamine Is A Fast Excitatory Transmitter At 5-HT3Receptors In Rat Amygdala,”Neuron, vol. 8, pp. 199-203 (1992).
Turton et al., “Antibodies Against The 5-HT3-A Receptor Identify A 54 kDa Protein Affinity-Purified From NCB20 Cells,”Mol. Neuropharmacol., vol. 3, pp. 167-171 (1993).
Van Hooft et al., “Phosphorylation Controls Conductance of 5-HT3Receptor Ligand-Gated Ion Channels,”Recept. Channels, vol. 3, pp. 7-12 (1995).
Voet et al., “Biochemistry,” John Wiley & Sons, Inc., pp. 126-129 and pp. 228-234 (1990).
Waeber et al., “5-HT3 Receptors In The Human Brain: Autoradiographic Visualization Using [3H]ICS 205-930,”Neuroscience, vol. 31(2), pp. 393-400 (1989).
Yakel et al., “Activation And Desensitization Of The 5-HT3Receptor In A Rat Glioma x Mouse Neuroblastoma Hybrid Cell,”J. Physiol. (London) vol. 436, pp. 293-308 (1991).
Zerr et al., “Episodic Ataxia Mutations in Kv1.1 Alter Potassium Channel Function By Dominant Negative Effects Or Haploinsufficiency,”J. Neuroscience, vol. 18(8), pp. 2842-2848 (1998).
Dubin et al., “The Pharmacological And Functional Characteristics Of The Serotonin 5-HT3AReceptor Are Specifically Modified By A 5-HT3BReceptor Subunit,”J. Bio. Chem., vol. 274(43), pp. 30799-30810 (1999).
Miquel et al., “Developmental Changes In the Differential Expression Of Two Serotonin 5-HT3Receptor Splice Variants In The Rat,”J. Neurochemistry, vol. 65(2), pp. 475-483 (1995).
Buehler Lukas K.
Dubin Adrienne E.
Erlander Mark G.
Huvar Arne
Huvar Rene
Andres Janet L.
Ballard Kimberly A.
Ortho-McNeil Pharmaceutical , Inc.
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