HSV helper virus independent vector

Details HSV helper virus independent vector HSV helper virus independent vector

1988-11-23

1990-11-13

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 691, 435320, 4352402, 935 29, 935 32, 935 34, 935 70, C12N 510, C12N 701, C12N 1538, C12N 1586

Patent

active

049701550

ABSTRACT:
This invention encompasses an eukaryotic expression vector constructed from a prokaryotic plasmid by inserting into the prokaryotic plasmid a mediator sequence and a promoter/regulatory sequence with a restriction endonuclease site for inserting a gene to be expressed. The gene to be expressed is inserted into the restriction endonuclease site in the promoter/regulatory sequence and the resulting plasmid is in turn used to transform a eukaryotic cell. The gene is expressed with or without viral mediation.

REFERENCES:
Stow, N. et al. in Eukaryotic Expression Vectors (ed Y. Gluzman), Cold Spring Harbor Laboratory, pp. 199-204, 1982.
Stow, N., The Embo Journal, vol. 1, No. 7, pp. 863-867, 1982.
Dubois, M., et al, Proc. Natl. Acad Sci, vol. 77, No. 8, pp. 4549-4553, 1980.
Burrell, C. J. et al., Nature, vol. 279, pp. 43-47, 1979.
Enquist, L. et al., Genevol. 7, pp. 335-342, 1979.
Post, Leonard E., et al., "A Generalized Technique for Deletion of Specific Genes in Large Genomes: .alpha.Gene 22 of Herpes Simplex Virus 1 is Not Essential for Growth," CELL, vol. 25, 227-232, Jul. 1981.

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