Host transformed with yeast gene and ubiquitin/polypeptide fusio

Details Host transformed with yeast gene and ubiquitin/polypeptide fusio Host transformed with yeast gene and ubiquitin/polypeptide fusio

1991-04-30

1993-11-16

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 697, 4351723, 435195, 435224, 4352523, 4353201, 536 232, 536 234, C12N 121

Patent

active

052623220

ABSTRACT:
A ubiquitin hydrolase is provided having a purity of at least 70% homogeneity based on the weight of the total protein in the composition. Also provided are DNA sequences encoding ubiquitin hydrolases, as well as expression systems for their recombinant production. Processes are provided for purification of a ubiquitin hydrolase from eukaryotes and for its use in recovering any desired polypeptide free from its fusion at its N-terminus with ubiquitin.

REFERENCES:
patent: 4757013 (1988-07-01), Inouye et al.
patent: 4771002 (1988-09-01), Gelvin
Bachmair et al., Science, 234:179-186 (1986).
Butt et al., PNAS USA, 86:2540-2544 (1989).
Hough et al., J. Biol. Chem., 261(5):2400-2406 (1986).
Mayer & Wilkinson, Biochemistry, 28:166-172 (1989).
Dverksen-Hughes et al., Biochemistry, 28:8530-8536 (1989).
Wilkinson et al., Science, 246:670-673 (1989).
Pickart and Rose, J. Biol. Chem, 260(13):7903-7910 (1985).
Pickart & Rose J. Biol. Chem 261(22):10210 (1986).
Rechsteiner, "Ubiquitin-Mediated Pathways for Intracellular Proteolysis," Ann. Rev. Cell Biol., 3:1-30 (1987).
Rechsteiner, M., Ubiquitin, Chapters 2, 5, and 6 (N.Y.: Plenium Press, 1988).
Ozkaynak, et al., "The yeast ubiquitin genes: a family of natural gene fusions", pp. 1429-1439, The EMBO Journal, 6: (1987).
Lund et al., "Nucleotide Sequence Analysis of a cDNA Encoding Human Ubiquitin Reveals that Ubiquitin is Synthesized as a Precursor," J. Biol. Chem., vol. 260, pp. 7609-7613 (1985).
Barr et al., "Production of Recombinant DNA-Derived Pharmaceuticals in the Yeast Saccharomyces Cerevisiae", Abst. 34, Amer. Chem. Soc., Sep. 25-30, 1988.
Kanda et al. (1986), "Substrate Recognition of Isopeptidase," BBA, vol. 870, 64-75.
Day et al. (1987), "Molecular cloning of cDNA coding for human PGP 9.5," FEB Letters, vol. 210, pp. 157-160.
Matsui et al. (1982), "Isopeptidase," PNAS, vol. 79, pp. 1535-1539.
Okayama et al. (1983), "A cDNA cloning vector," MCB, vol. 3, pp. 280-289.
Wood (1987), "Gene Cloning Based on Long Oligonucleotide Probes," in Methods in Enzynology, vol. 152 (Academic Press, N.Y.), pp. 443-447.
Scopes, Robert K. Protein Purification, 1987, Springer-Verlag, New York, pp. 100-107, 175-177.
Pickart and Rose, "Ubiquitin Carboxyl-terminal Hydrolase acts on Ubiquitin . . . Amides", Journ. Biol. Chem. 260, 13, Jul. 5, 1985.

Affiliated with

Also associated with

Rating

Host transformed with yeast gene and ubiquitin/polypeptide fusio does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Host transformed with yeast gene and ubiquitin/polypeptide fusio, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Host transformed with yeast gene and ubiquitin/polypeptide fusio will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-21836