Host expressing NgoAIII restriction endonuclease and modificatio

Details Host expressing NgoAIII restriction endonuclease and modificatio Host expressing NgoAIII restriction endonuclease and modificatio

1990-06-08

1992-09-15

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4353201, 435196, 435871, C12N 121, C12N 1574

Patent

active

051478005

ABSTRACT:
The present invention is directed to recombinant hosts which contain and express various Type II restriction endonuclease and/or modification methylase genes. In particular, the present invention is concerned with the cloned restriction endonucleases, NgoAIII and NgoAI, which recognize and cleave within or near the double-stranded DNA sequence, 5' CCGCGG 3' and 5' PuGCGCPy 3', respectively. Also provided in this invention are cloned modification methylase genes corresponding to said restriction endonucleases. This invention is further concerned with a cloned modification methylase, M.NgoAII. One source of these enzymes is Neisseria gonorrhoeae, although other microorganisms may be used to isolate the restriction endonuclease isoschizomers and modification methylase isoschizomers of this invention.

REFERENCES:
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Davies Clin. Microbiol. Res. Apr. 1989, p. 578.
Chien et al., Cloning and Characterization of a Restriction and Modification System from Neisseria gonorrhoeae Strain MS11, Abstracts of the ASM Annual Meeting, p. 213 (1988).
Roberts, R. J., Restriction Enzymes and Their Isoschizomers, Nucleic Acids Research, 17, Supp., pp. 347-387.
Sullivan, K. M. and Saunders, J. R., Sequence Analysis of the NgoPII Methyltransferase Gene from Neisseria gonorrhoeae P9 . . . , Nucleic Acids Research, 16(10): 4369-4387, (1988).
Sullivan et al., Characterization of DNA Restriction and Modification Activities in Neisseria Species, FEMS Microbiology Letters, 44:389-393 (1987).
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New England Biolabs, 1988-1989 Catalog, pp. 19 and 29.

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