Host cells for production of isoprenoid compounds

Details Host cells for production of isoprenoid compounds Host cells for production of isoprenoid compounds

2011-03-29

2011-03-29

Fronda, Christian L (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S041000, C435S132000, C435S183000, C435S189000, C435S194000, C435S232000, C435S320100

Reexamination Certificate

active

07915026

ABSTRACT:
Methods for synthesizing isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. A related method comprises introducing into a host microorganism an intermediate in the mevalonate pathway and at least one heterologous nucleic acid sequence, each sequence coding for an enzyme in the mevalonate pathway necessary for converting the intermediate into isopentenyl pyrophosphate. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods.

REFERENCES:
patent: 6072045 (2000-06-01), Chappell et al.
patent: 6114160 (2000-09-01), Croteau et al.
patent: 6190895 (2001-02-01), Croteau et al.
patent: 6281017 (2001-08-01), Croteau et al.
patent: 6284506 (2001-09-01), Hoshino et al.
patent: 6291745 (2001-09-01), Meyer et al.
patent: 6306633 (2001-10-01), Wilding et al.
patent: 6495354 (2002-12-01), Chappell et al.
patent: 6531303 (2003-03-01), Mills et al.
patent: 6916972 (2005-07-01), Falco et al.
patent: 6989257 (2006-01-01), Berry et al.
patent: 7129392 (2006-10-01), Hahn et al.
patent: 7132268 (2006-11-01), Miyake et al.
patent: 2004/0029239 (2004-02-01), Ohto et al.
patent: 2004/0063182 (2004-04-01), Ohto et al.
patent: 2005/0241017 (2005-10-01), Hahn et al.
patent: 2005/0266518 (2005-12-01), Berry et al.
patent: 2007/0077616 (2007-04-01), Keasling et al.
patent: 0955363 (1999-11-01), None
patent: 1360300 (2003-11-01), None
patent: 1392824 (2004-03-01), None
patent: WO 0210398 (2002-02-01), None
patent: WO 02099095 (2002-12-01), None
patent: WO 0001650 (2005-01-01), None
Altincicek et al., “GcpE is Involved in the 2-C-Methyl-D-Erythritol 4-Phosphate Pathway of Isoprenoid Biosynthesis inEscherichia coli” (2001) Journal of Bacteriology, vol. 183, No. 8, pp. 2411-2416.
Amann et al., Tightly Regulated Tac Promoter Vectors Useful for the Expression of Unfused and Fused Proteins inEscherichia coli, (1988), Gene, vol. 69, pp. 301-315.
Balbas et al., (1996), A pBRINT family of plasmids for integration of cloned DNA into theEscherichia colichromosome. Gene, vol. 172, No. 1, pp. 65-69.
Barkovich et al., “Metabolic Engineering of Isoprenoids” (2001), Metabolic Engineering, vol. 3, No. 1, pp. 27-39.
Campos et al., Identification of gcpE as a Novel Gene of the 2-C-Methyl-D-Erythritol 4-Phosphate Pathway for Isoprenoid Biosynthesis inEscherichia coli, (2001), FEBS Letters, vol. 488, pp. 170-173.
Campos et al.,Escherichia coliEngineered to Synthesize Isopentenyl Diphosphate and Dimethylallyl Diphosphate from Mevalonate: A Novel System for the Genetic Analysis of the 2-C-Methyl-D-Erythritol 4-Phosphate Pathway for Isoprenoid Biosynthesis (2001) Biochemistry Journal, vol. 353, pp. 59-67.
Cunningham et al., “Molecular Structure and Enzymatic function of Lycopene Cyclase from theCyanobacterium synechococcussp Strain PCC7942,” (1994) The Plant Cell, vol. 6, pp. 1107-1121.
Dairi et al., Eubacterial Diterpene Cyclase Genes Essential for Production of the Isoprenoid Antibotic Terpentecin (2001), Journal of Bacteriology, vol. 183, No. 20, pp. 6085-6094.
Guzman et al, Tight Regulation, Modulation, and High-Level Expression by Vectors Containing the Arabinose PBADPromoter, (1995), Journal of Bacteriology, vol. 177, No. 14, pp. 4121-4130.
Hahn et al., “Escherichia coliOpen Reading Frame 696 Is idi, a Nonessential Gene Encoding Isopentenyl Diphosphate Isomerase,” (1999), Journal of Bacteriology, vol. 181, No. 15, pp. 4499-4504.
Hahn et al, “I-Deoxy-D-Xylulose 5-Phosphate Syntheses, the Gene Product of Open Reading Frame (ORF) 2816 and ORF 2895 inRhodobacter capsulatus” (2001), Journal of Bacteriology, vol. 183, No. 1, pp. 1-11.
Hamano et al., Cloning of a Gene Cluster encoding Enzymes Responsible for the Mevalonate Pathway from a Terpenoid-Antibioltic-ProducingStreptomycesStrain, (2001), Biosci Biotechnol. Biochem. vol. 65, No. 7, pp. 1627-1635.
Kaneda et al., “An Unusual Isopentenyl Diphosphase Isomerase Found in the Mevalonate Pathway Gene Cluster fromStreptomycessp. Strain CL190” (2001), PNAS, vol. 98, No. 3, pp. 932-937.
Kim et al., “Metabolic Engineering of the Nonmevalonate Isopentenyl diphosphate Synthesis Pathway inEscherichia coliEnhances Lycopene Production” (2001), Biotechnology and Bioengineering, vol. 72, No. 4, pp. 408-415.
Kovach et al., “pBBRIMCS: A Broad-Host-Range Cloning Vector” (1994), BioTechniques, vol. 16, No. 5, pp. 800-802.
Kovach et al., Four New Derivatives of the Broad-Host-Range-Cloning Vector pBBRIMCS. Carrying Different Antibiotic-Resistance Cassettes, (1995), Gene, vol. 166, pp. 175-176.
Mahmoud et al., “Metabolic Engineering of Essential Oil Yield and Composition in Mint by Altering Expression of Deoxyxylulose Phosphate Reductoisomerase and Methofuran Synthase”, (2001), PNAS, vol. 98, No. 15, pp. 8915-8920.
McAteer et al., “The lytb Gene ofEscherichia coliIs Essential and Specifies a Product Needed for Isoprenoid Biosyntheis” (2001), Journal of Bacteriology, vol. 183, No. 24, pp. 7403-7407.
Oulmouden et al., “Nucleotide Sequence of the ERG12 Gene ofSaccharomyces cerevisaeEncoding Mevalonate Kinase” (1991), Current Genetics, vol. 19, pp. 9-14.
Polakowski et al., “Overexpression of a Cytosolic Hydroxymethylglutaryl-CoA Reductase Leads to Squalene Accumulation in Yeast”, (1998), Appl. Microbiol. Biotechnol., vol. 49, pp. 66-71.
Rohdich et al., Studies on the Nonmevalonate Terpene Biosynthetic Pathway: Metabolic Role of IspH (LyB) Protein, (2002), PNAS, vol. 99, No. 3, pp. 1158-1163.
Rohlin et al., “Microbioal Pathway Engineering for Industrial Processes: Evolution, Combinatorial Biosynthesis and Rational Design” (2001), Current Opinion in Microbiology, vol. 4, pp. 330-335.
Rohmer et al., “Isoprenoid Biosynthesis in Bacteria: A Novel Pathway for the Early Steps Leading to Isopentenyl Diphosphate”, (1993), Biochem. J., vol. 295, pp. 517-524.
Sandmann, “Carotenoid Biosynthesis and Biotechnological Application” (2001), Archives of Biochemistry and Biophysics, vol. 385, pp. 4-12.
Szkopinska et al., “The Regulation of Activity of Main Mevalonic Adic Pathway Enzymes: Farnesyl Diphosphas Synthase. 3-Hydroxy-3-Methylglutaryl-CoA Reductase, and Squalene Synthase in YeastSaccharomyces cerevidiae”, (2000), Biochemical and Biophysical Research Communications, vol. 267, pp. 473-477.
Takagi et al., “A Gene Cluster for the Mevalonate Pathway fromStreptomycessp. Strain CL190.” (2000), Journal of Bacteriology, vol. 182, No. 15, pp. 4153-4157.
Tatiana et al., FEMS Microbiol Lett, May 15, 1999, vol. 174, No. 2, pp. 247-250.
Toth et al., “Molecular Cloning and Expression of the cDNAs Encoding Human and Yeast Mevalonate Pyrophosphate Decarboxylase”, (1996), The Journal of Biological Chemistry, vol. 271, No. 14, pp. 7895-7898.
Tsay et al., “Cloning and Characterization of ERG8, an Essential Gene ofSaccharomyces cervisiaethat Encodes Phosphomevalonate Kinase”, (1991), Molecular and Cellular Biology, vol. 11, No. 2, pp. 620-631.
Wang et al., “Engineered Isoprenoid Pathway Enhances Astaxanthin Production inEscherichia coli”, (1999), Biotechnology and Bioengineering, vol. 62, No. 2, pp. 235-241.
Wang et al., Directed Evolution of Metabolically EngineeredEscherichia colifor Carotenoid Production, (2000), Biotechnol. Prod., vol. 16, No. 6, pp. 922-926.
66 FR 1099, Friday, Jan. 5, 2001.
BLAST 2 Sequences results. Sequence 1 gi 5531936, Sequence 2 gi 546900, printed on Jun. 24, 2005.
Hiser et al., “ERG10 fromSaccharomyces cerevisiaeEncodes Acetoacetyl-CoA Thiolase” (1994), Journal of Biological Chemistry, vol. 269, No. 50, pp. 31383-31389.
Hiser et al., Accession L20428, Feb. 23, 1995.
National Science Foundation Award Abstract No. 9911463.
Fujisaki et al

Affiliated with

Also associated with

Rating

Host cells for production of isoprenoid compounds does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Host cells for production of isoprenoid compounds, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Host cells for production of isoprenoid compounds will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2699950