Host cell modifications that improve peptide production and...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S252330

Reexamination Certificate

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07915011

ABSTRACT:
Disrupting the expression of endogenousEscherichiahost cell genes gcvA and spr provides mutant host cells having increased heterologous peptide production. The addition of a genetic modification to the coding region of gene yejM further enhances peptide production and facilitates easier downstream processing. RecombinantEscherichiahost cells are provided as well as methods of using such host cells for heterologous peptide production.

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