Host cell expressing reduced levels of a metalloprotease and met

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 694, 435 6951, 435 696, 435189, 435198, 435212, 435484, C12P 2102

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059687746

ABSTRACT:
The present invention relates to novel host cells and to methods of producing proteins. More specifically the invention relates to a host cell useful for the expression of heterologous proteins, which host cell has been genetically modified in order to express significantly reduced levels of a metalloprotease. Moreover the invention relates to a method of producing a heterologous protein, which method comprises cultivating the host cell in a suitable growth medium, followed by recovery of the desired protein.

REFERENCES:
Tatsumi et al., "Cloning And Expression In Yeast Of A cDNA Clone Encoding Aspergillus oryzae Neutral Protease II, A Unique Metalloprotease", Mol Gen Genet, 1991, 228: pp. 97-103.

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