Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Patent
1995-06-07
1998-10-13
Rollins, John W.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
435366, 435363, 435404, 435 701, 435 703, 435 41, C12N 500, C12P 2104, C12P 100
Patent
active
058211217
ABSTRACT:
Methods are provided for the establishment and maintenance in long term culture of hormone secreting cells. Cells are derived from animal or human tissues, including ovary, endometrium, trophoblast, pituitary, thyroid, and pancreas. Pancreatic cells are obtained by gently rupturing the basement lamina of a whole islet. The pancreatic cells that develop through the rupture in the basement lamina are cultured. The cells secrete into the culture medium various hormones depending on the tissue of origin of individual cell cultures. Contact with an appropriate secretogogue causes the cells to respond with increased hormone secretion. For instance, pancreatic cells respond to elevated glucose with increased insulin secretion. The cells proliferate in vitro for up to one year or longer, during which time they retain their hormone-secretion profile. The cells may be frozen for storage, and retain their hormone-secretion profile after thawing. The cell cultures are useful for the production of human hormones, for the bio-assay of drugs, for the testing of drug efficacy and design, and for toxicity testing of drugs and chemicals. The cells may also be implanted in an individual to replace deficient hormone secretion.
REFERENCES:
patent: 4657866 (1987-04-01), Kumar
patent: 4929542 (1990-05-01), Risley
patent: 4980281 (1990-12-01), Housey
patent: 4983515 (1991-01-01), Maley et al.
patent: 4983527 (1991-01-01), Capco et al.
patent: 5232848 (1993-08-01), Wolfe et al.
patent: 5328844 (1994-07-01), Moore
ATCC CCL 98; May 1990 Supplement to 1988 American Tissue Culture collection (ATCC) catalog of cell lines, p. 61.
ATCC HTB 161; May 1990 Supplement to 1988 American Tissue Culture Collection (ATCC catalog of cell lines, p. 244.
Baum, G., et al., "Regulation of Tropomyosin Expession in Transformed Granulosa Cell Lines with Steroidogenic Ability," Develop Biol., 112:115-128 (1990).
Amsterdam, A., et al., "Synergistic Effect of Human Chorionic Gonadotropin and Extracellular Matrix on in vitro Differentiation of Human Granulosa Cells: Progesterone Production and Gap Junction Formation," Endocrinology, 124:1956-1964 (1989).
Pellicer, A., et al., "Steroidogenesis in vitro of human granulosa-luteal cells pretreated in vivo with gonadotropin-releasing hormone analogs," Fertility and Sterility 54:590-596 (1990).
Vanderhyden, B.C., et al., "Developmental Pattern of the Secretion of Cumulus Expansion-Enabling Factor by Mouse Oocytes and the Role of Oocytes in Promoting Granulosa Cell Differentiation," Develop. Biol. 140:307-317 (1990).
Nau, H., "Pharmacokinetic Aspects of In Vitro Teratogenicity Studies: Comparison to In Vivo," Methods in Developmental Toxicology: Use in Defining Mechanisms and Risk Parameters. eds., G.L. Kimmel, D.M. Kochhar, CRC Press, pp. 37-43 (1990).
Tsushimoto, G., et al., "Cytotoxic, Mutagenic, and Cell-Cell Communication Inhibitory Properties of DDT, Lindane, and Chlordane on Chinese Hamster Cells in vitro," Arch. Environ. Contam. Toxicol. 12:721-730 (1983).
Aaladjem, S., et al., "Observations of Trophoblast from Normal and Pre-clamptic Pregnancies Cultured for Prolonged Periods," Placenta Suppl. 3:175-179 (1981).
Nau, H., et al., "Weak acids may act as teratogens by accumulating in the basic milieu of the early mammalian embryo," Nature 323:276-279 (1986).
Hamilton, T.C., et al., "Characterization of a Xenograft Model of Human Ovarian Carcinoma Which Produces Ascites and Intraabdominal Carcinomatosis in Mice," Cancer Research 44:5286:5290 (1984).
Pattillo, R.A., et al., "Human Hormone Production in vitro," Science 159:1467-1469 (1968).
Hertz, R., "Choriocarcinoma of Women Maintained in Serial Passage in Hamster and Rat," Proc. Soc. Exp. Biol. Med. 102:77-80 (1959).
Pattillo, R.A., et al., "Control Mechanisms for Gonadotrophic Hormone Production In Vitro," In Vitro 6:205-214 (1970).
Pattillo, R.A., et al., "The Hormone-Synthesizing Trophoblastic Cell In Vitro: A Model for Cancer Research and Placental Hormone Synthesis," Ann. N.Y. Acad. Sci. 172:288-298 (1971).
Pattillo, R.A., et al., "Estrogen Production by Trophoblastic Tumors in Tissue Culture," J. Clin. Endocrinol. Metab. 34:59-61 (1972).
Jia, X.-C., et al., "Granulosa Cell Aromatase Bioassay for Follicle-Stimulating Hormone: Validation and Application of Method," Endocrinology 119:1520-1577 (1985).
Van Damme, M.-P., et al., "A Sensitive and Specific In Vitro Bioassay Method for the Measurement of Follicle-Stimulating Hormone Activity," Acta Endocrinol. (Copen) 91:224-237 (1979).
Doody, K.H., et al., "Regulation of 3.beta.-Hydroxysteroid Dehydrohgenase and Cholesterol Side Chain Cleavage Cytochrome P-450 Expession by Forskolin and Transforming Growth Factor .beta. (TGF.beta.) in Lutenized Human Granulosa Cells," Soc. Gyn. Invet. 38th Annual Mtg., Abstract 431 (1991).
Daneshdoost, L., et al., "Recognition of Gandotroph Adenomas in Women," New England J. Med. 324:589-594 (1991).
Wier, P.J., et al., "In Vitro Evaluations of Human Placental Functions and Toxic Responses," In Vitro Methods in Developmental Toxicology, G.L. Kimmel and D.M. Kochar, eds., CRC Press, Boca Raton, pp. 11-28 (1990).
Skinner, M.K., et al., "Developmental and Hormonal Regulation of Bovine Granulosa Cell Function in the Preovulatory Follicle," Endocrinol. 121:786-792 (1987).
Skinner, M.K., et al., "Ovarian Thecal Cells Produce Transforming Growth Factor-.beta. Which Can Regulate Granulosa Cell Growth," Endocrinol. 123:1668-1675 (1988).
White, T., et al., "Human Choriocarcinoma (JAr) Cells Grown as Multicellular Spheroids," Placenta 9:583-596 (1988).
Kohler, P.O., et al., "Isolation of Hormone-Producing Clonal Lines of Human Choriocarcinoma," J. Clin. Endocrinol. Metab. 32:683-695 (1971).
Chou, J.Y., "Establishment of clonal human placental cells synthesizing human choriogonadotropin," Proc. Natl. Acad. Sci. (USA) 75:1854-1858 (1978).
Dorrington, J.H., et al., "Estradiol-17.beta. Biosynthesis in Cultured Granulosa Cells from Hypophysectomized Immature Rats: Stimulation by Follicle-Stimulating Hormone," Endocrinol. 97:1328-1331 (1975).
Stone, B.A., et al., "Between-lot variability in chromatographic and biochemical properties of hMG," Acta Endo. (Copenhagen) 123:161-168 (1990).
Ng, K.W., et al., "Insulin release from a cloned precursor beta cell line," J. Endocr. 113:3-10 (1987).
Santerre, R.F., et al., "Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells," Proc. Natl. Aca. Sci. USA 78:4339-4343 (1981).
Clark, S.A., et al., "Modulation of Glucose-Induced Insulin Secretion from a Rat Clonal .beta.-Cell Line," Endocrinology 127:2779-2788 (1990).
Pattillo, R.A., et al., "Hormone Synthesis and Function In Vitro," Growth, Nutr. Metabl. Cell Cult. 2:213-249 (1972).
Freshney, I., Culture of Animal Cells, 3rd Edition, A Manual of Basic Techniques, Wiley, eds., p. 70 (1989).
Pattillo, R.A., et al., "The Establishment of a Cell Line of Human Hormone-synthesizing Trophoblastic Cells in Vitro," Cancer Res. 28:1231-1236 (1968).
Budd, G.C., et al., "Detection of Insulin Synthesis in Mammalian Anterior Pituitary Cells by Immunohistochemistry and Demonstration of Insulin-Related Transcripts by in situ RNA-DNA Hybridization," J. Histochem. and Cytochem. 34:673-678 (1986).
Takaki, R., "Culture of Pancreatic Islet Cells and Islet Hormone Producing Cell Lines `Morphological and Functional Integrity in Culture`" In Vitro Cell and Develop. Biol. 25(9):763-769 (1989).
Tilly, J.L., et al., "Regulation of Androstenedione Production by Adenosine 3',5'-Monophosphate and Phorbol Myristate Acetate in Ovarian Thecal Cells of the Domestic Hen," Endocrinology 125:1691-1699 (1989).
Pacific Biomedical Research, Inc.
Rollins John W.
Smith Karen S.
Tate Christopher R.
LandOfFree
Hormone-secreting cells maintained in long-term culture does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Hormone-secreting cells maintained in long-term culture, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Hormone-secreting cells maintained in long-term culture will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-312646