Horizontal slab gel electrophoresis

Details Horizontal slab gel electrophoresis Horizontal slab gel electrophoresis

1978-01-30

1979-04-24

Prescott, Arthur C.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

204180G, 23230B, 424 12, G01N 2726, G01N 2728

Patent

active

041510658

ABSTRACT:
An improved horizontal slab gel electrophoresis apparatus having compartments for vertical wicks at each end of a removable horizontal trough. Adjacent to the vertical wicks are vertical buffer compartments with built in electrodes. A partition separates the vertical wirks from the buffer compartment and includes a slot with a silicone plug which can be removed after curing or hardening of the vertical wicks to permit communication between the buffer and the gel slab. Flow adapters and an interconnecting tube permit constant circulation of the buffer to maintain equal volume and pH. The horizontal trough has slots for insertion of combs to form up to seventy-five sample wells and additional slots at each end for sealing the end of the horizontal slab when the gel slab is removed. The apparatus includes coils in a horizontal bed or plate beneath the trough for cooling the gel slab during a run and levelling screws for levelling the horizontal trough before pouring the gel slab.

REFERENCES:
patent: 3773647 (1973-11-01), Mandel et al.
patent: 3873433 (1975-03-01), Seidel et al.

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