Horizontal polyacrylamide gel electrophoresis

Details Horizontal polyacrylamide gel electrophoresis Horizontal polyacrylamide gel electrophoresis

1992-08-28

1994-08-16

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

C25B 900

Patent

active

053384261

ABSTRACT:
An electrophoresis apparatus has a gel film cast between two plates and buffer reservoirs at each end of the film with electrodes connectable to an external power supply for providing electromotive force for driving electrophoresis. The reservoirs are configured to wet the ends of the gel film and submerge the electrodes with the apparatus positioned either horizontally or vertically, so gel films can be cast horizontally with sample wells formed in the end of the gel between the plates. Samples may be added to the wells and run into the gel with the apparatus positioned vertically, and the analytical separation may be performed with the apparatus again positioned horizontally, such as in an automatic scanning apparatus.

REFERENCES:
patent: 3719580 (1973-03-01), Roberts et al.
patent: 5137613 (1992-08-01), Brumley, Jr. et al.
El-Negoumy, "A convenient migration chamber for electrophoresis in solid gels." J. Chromatog. 23 (1966) 325-329.

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