Homologously recombinant slow growing mycobacteria and uses ther

Details Homologously recombinant slow growing mycobacteria and uses ther Homologously recombinant slow growing mycobacteria and uses ther

1995-06-07

2000-02-08

Brusca, John S.

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

4351736, 4352523, 4352531, 536 235, 536 237, C12N 1564, C12N 1300, C12N 121, C12N 1531

Patent

active

060227451

ABSTRACT:
A method of transforming slow-growing mycobacteria, such as M. bovis BCG, M. leprae, M. tuberculosis M. avium, M. intracellulare and M. africanum; a method of manipulating genomic DNA of slow-growing mycobacteria through homologous recombination; a method of producing homologously recombinant (HR) slow-growing mycobacteria in which heterologous DNA is integrated into the genomic DNA at a homologous locus; homologously recombinant (HR) slow-growing mycobacteria having heterologous DNA integrated into their genomic DNA at a homologous locus; and mycobacterial DNA useful as a genetic marker.

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