Homologously recombinant slow growing mycobacteria and uses ther

Details Homologously recombinant slow growing mycobacteria and uses ther Homologously recombinant slow growing mycobacteria and uses ther

1995-05-19

1999-02-02

Degen, Nancy

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4351723, 4352531, 4353201, 536 231, 536 235, 536 237, C12N 121, C12N 1563, C12N 2104

Patent

active

058664033

ABSTRACT:
A method of transforming slow-growing mycobacteria, such as M. bovis BCG, M. leprae, M. tuberculosis M. avium, M. intracellulare and M. africanum; a method of manipulating genomic DNA of slow-growing mycobacteria through homologous recombination; a method of producing homologously recombinant (HR) slow-growing mycobacteria in which heterologous DNA is integrated into the genomic DNA at a homologous locus; homologously recombinant (HR) slow-growing mycobacteria having heterologous DNA integrated into their genomic DNA at a homologous locus; and mycobacterial DNA useful as a genetic marker.

REFERENCES:
patent: 4906742 (1990-03-01), Young et al.
patent: 4910140 (1990-03-01), Dower
patent: 4952500 (1990-08-01), Finnerty et al.
Botstein et al. Principles and practice of recombinant DNA research with yeast. in The Molecular Biology of the Yeast Saccharomyces Metabolism and Gne Expression Strathern, Jones, Broach, Eds. Cold Spring Harbor Laboratory pp. 607-636, 1982.
Norgard et al. Physiological factors involved in the transformation of Mycobacterium smegmatis. J. Bacteriology vol. 133 pp. 1254-1262, 1978.
Ramakrishnan, T. and M.S. Shaila, "Interfamilial Transfer of Amber Suppressor Gene for the Isolation of Amber Mutants of Mycobacteriophage I3", Arch. Microbiol., 120:301-302 (1979).
Jacobs, W.R. et al., "Expression of Mycobacterium leprae Genes From a Streptococcus mutans Promoter in Escherichia coli K-12", Proc. Natl. Acad. Sci. USA, 83:1926-1930 (1986).
Husson, R.N. et al., "Genes for the Major Protein Antigens of Mycobacterium tuberculosis . . . ", Proc. Natl. Acad. Sci. USA, 84:1679-1683 (1987).
Shinnick, T.M. et al., "The Etiologic Agents of Leprosy and Tuberculosis Share an Immunoreactive Protein Antigen . . . ", Infection & Immunity, 55(8):1932-1935 (1987).
Lu, M.C. et al., "Genes for Immunodominant Protein Antigens Are Highly Homologous . . . ", Infection & Immunity, 55:2378-2382 (1987).
Lamb,F.I. et al. "Heterologous Expression of the 65-Kilodalton Antigen of Mycobacterium leprae and Murine T-Cell Responses to the Gene Product", Infection & Immunity, 56:1237-1241 (1988).
Sirakova, T.D. et al., "Molecular Cloning of Mycobacterial Promoters in Escherichia coli", FEMS Micro. Lett., 59:153-156 (1989).
Stoker, N.G. et al., "High Level Expression of Genes Cloned in Phage .lambda.gt11", Gene, 78:93-99 (1989).
Borremans, M. et al., "Cloning, Sequence Determination, and Expression of a 32-Kilodalton-Protein Gene of Mycobacterium tuberculosis", Infection & Immunity, 57:3123-3130 (1989).
Husson, R.N. et al., "Gene Replacement and Expression of Foreign DNA in Mycobacteria", J. Bacteriol., 172:519-524 (1990).
Snapper, S.B. et al., "Lysogeny and Transformation in Mycobacteria: Stable Expression of Foreign Genes", Proc. Natl. Acad. Sci. USA, 85:6987-6991 (1988).
Vodkin, M.H. et al., "A Heat Shock Operon in Coxiella burnetii Produces a Major Antigen Homologous to a Protein in Both Mycobacteria and Escherichia coli", J. Bacteriol. 170:1227-1234 (1988).
Baird, P.N. et al., "Cloning and Sequence Analysis of the 10 kDa Antigen Gene of Mycobacterium tuberculosis", J. Gen. Microbiol., 135:931-939 (1989).
Hone, D. et al., "A Chromosomal Integration System for Stabilization of Heterologous Genes in Salmonella Based Vaccine Strains", Microbial Pathogenesis, 5:407-418 (1988).
Mackett, M. et al., "General Method for Production and Selection of Infectious Vaccinia Virus Recombinants Expressing Foreign Genes", J. Virol., 49:857-864 (1984).
Clements, J.D. et al., "Construction of a Potential Live Oral Bivalent Vaccine for Typhoid Fever and Cholera-Escherichia coli-Related Diarrheas", Infection & Immunity, 46:564-569 (1984).
Young, R.A.et al., "Dissection of Mycobacterium tuberculosis Antigens Using Recombinant DNA", Proc. Natl. Acad. Sci. USA, 82:2583-2587 (1985).
Lindquist, S. et al., "The Heat-Shock Proteins", Ann. Rev. Genet., 22:631-677 (1988).
Lathigra, R.B. et al., "A Gene From Mycobacterium tuberculosis Which Is Homologous to the DnaJ Heat Shock Protein of E. coli", Nucleic Acids Res., 16:1636 (1988).
Burke, J.F., "An Assay for Transient Gene Expression in Transfected 3:2549-2554 (1984).
Suarez, J.E. et al., "DNA Cloning in Streptomyces . . . ", Nature, 286:527-529 (1980.
Post, L.E. et al., "A Generalized Technique for Deletion of Specific Genes in Large Genomes . . . ", Cell, 25:227-232 (1981).
Crawford, J.T. et al.., "Characterization of Plasmids From Strains of Mycobacterium avium-intracellulare", Rev. Infec. Diseases, 3(5):949-952 (1981).
Lotte, A. et al., "BCG Complications; Estimates of the Risks . . . ", Adv. in Tuberculosis Res., 21:107-193 (1984).
Labidi, A. et al., "Plasmid Profiles of Mycobacterium fortuitum Complex Isolates", Current Microbiol., 11:235-240 (1984).
Crawford, J.T. et al., "Restriction Endonuclease Mapping and Cloning of Mycobacterium intracellulare plasmid pLR7", Gene, 27:331-332 (1984).
Labidi, A. et al., "Cloning and Expression of Mycobacterial Plasmid DNA in Escherichia coli", FEMS Microbiol. Lett., 30:221-225 (1985).
Labidi, A. et al., "Restriction Endonuclease Mapping and Cloning of Mycobacterium Fortuitum var. Fortuitum Plasmid pAL5000", Ann. Inst. Pasteur/Microbiol., 136B:209-215 (1985).
Crawford, J.T. et al., "Analysis of Plasmids in Mycobacterium avium-intracellulare Isolates from Persons With Acquired Immunodeficiency Syndrome", Am. Rev. Respir. Dis., 134:659-661 (1986).
Jacobs, W.R. et al., "Introduction of Foreign DNA Into Mycobacteria Using a Shuttle Plasmid", Nature, 327:532-535 (1987).
Jacobs, W.R. et al., "In Vivo Repackaging of Recombinant Cosmid Molecules for Analyses of Salmonella typhimurium, Streptococcus mutans, and Mycobacterial Genomic Libraries", Infection & Immunity, 52:101-109 (1986).
Timme, T.L. et al., "Induction of Bacteriophage from Members of the Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum Serocomplex", J. Gen. Microbiol., 130:2059-2066 (1984).
Young, D.B. et al., "Leprosy, Tuberculosis and the New Genetics", J. Bacteriol., 175:1-6 (1993).
Kalpana, G.V. et al., "Insertional Mutagenesis and Illegitimate Recombination in Mycobacteria", Proc. Natl. Acad. Sci. USA, 88:5433-5437 (1991).
Hermans, J. et al., "Transformation of Mycobacterium aurum by electroporation: the use of glycine, lysozyme and isonicotinic acid hydrazide in enhancing transformation efficiency," FEMS Microbiology Letters 72:221-224 (1990).
Jacobs, William R., Jr. et al., "Genetic Systems for Mycobacteria," Methods in Enzymology 204:537-555 (1991).
Anna Aldovini et al., "The uraA Locus and Homologous Recombination in Mycobacterium bovis BCG," Journal of Bacteriology 175(22):7282-7289 (1993).
Lee, M.H., et al., "Site-specific integration of mycobacteriophage L5: Integration-proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-Guerin," Proc. Natl. Acad. Sci. USA 88: 3111-3115 (1991.

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