Homologous recombination in mismatch repair inactivated...

Details Homologous recombination in mismatch repair inactivated... Homologous recombination in mismatch repair inactivated...

2007-04-03

2007-04-03

Woitach, Joseph (Department: 1632)

Multicellular living organisms and unmodified parts thereof and

Method of making a transgenic nonhuman animal

C800S018000, C800S025000, C435S325000

Reexamination Certificate

active

09884877

ABSTRACT:
A mammalian cell having a mismatch repair-deficient phenotype is provided, where one or both alleles of a gene essential for mismatch repair, such as an Msh gene, are inactivated. Using this cell in a gene knock-out methodology advantageously allows efficient homologous recombination, even when the DNA sequences of the donor and recipient sequences diverge by significantly more than 0.6%.

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