Homologous 28-kilodalton immunodominant protein genes of...

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C424S234100, C435S069300, C435S069700, C435S091200, C435S326000, C435S340000, C536S023500, C536S023700, C514S002600, C514S012200

Reexamination Certificate

active

10731554

ABSTRACT:
The present invention is directed to the cloning, sequencing and expression of homologous immunoreactive 28-kDa protein genes, p28-1, -2, -3, -5, -6, -7, -9, from a polymorphic multiple gene family ofEhrlichia canis. Further disclosed is a multigene locus encoding all nine homologous 28-kDa protein genes ofEhrlichia canis. RecombinantEhrlichia canis28-kDa proteins react with convalescent phase antiserum from anE. canis-infected dog, and may be useful in the development of vaccines and serodiagnostics that are particularly effective for disease prevention and serodiagnosis.

REFERENCES:
patent: 3250086 (1966-05-01), Morris, Jr.
patent: 3368363 (1968-02-01), Alaburda et al.
patent: 3410101 (1968-11-01), Morris, Jr.
patent: 5184471 (1993-02-01), Losacco et al.
patent: 5329842 (1994-07-01), Zittel
patent: 5429041 (1995-07-01), Zittel
patent: 5456091 (1995-10-01), Zittel
patent: 5509470 (1996-04-01), Bass
patent: 5531034 (1996-07-01), Mentz
patent: 5752431 (1998-05-01), Zittel
patent: 5868000 (1999-02-01), Morris, Jr. et al.
patent: 6043085 (2000-03-01), Yu et al.
patent: 6161613 (2000-12-01), Huenniger
patent: 6214400 (2001-04-01), Zittel et al.
patent: 6234066 (2001-05-01), Zittel et al.
patent: 6263785 (2001-07-01), Zittel
patent: 6308529 (2001-10-01), Bass
patent: 6392023 (2002-05-01), Walker et al.
patent: 6403780 (2002-06-01), Walker et al.
patent: 6658886 (2003-12-01), Bass
patent: 6660269 (2003-12-01), Walker et al.
patent: WO 98/16554 (1998-04-01), None
patent: WO 99/13720 (1999-03-01), None
patent: WO 00/32745 (2000-06-01), None
Uniprot—05, Accession No. Q9ADV2—EHRCA.
U.S. Appl. No. 60/059,353, filed Sep. 19, 1997, Rikihisa et al.
Anderson et al., “Ehrlichia chaffeensis, a new species associated with human ehrlichiosis,”J Clin Microbiol, 29(12):2838-2842, 1991.
Anderson et al., “Ehrlichia ewingiisp. Nov., the etiologic agent of canine granulocytic ehrlichiosis”Int J Syst Bacteriol, 42(2):299-302, 1992.
Brouqui et al., “Antigenic characterization of ehrlichiae: protein immunoblotting ofEhrlichia canis, Ehrlichia sennetsu, andEhrlichia risticii,” J Clin Microbiol, 30(5):1062-1066, 1992.
Burgess et al., “Possible dissociation of the heparin-binding and mitogenic activites of heparin-binding (acidic fibroblast) growth factor-1 from its receptor-binding activities by site-directed mutagenesis of a single lysine residue,”J. Cell. Biol., 111:2129-2138, 1990.
Chen et al., “Identification of the antigenic constituents ofEhrlichia chaffeensis,” Am J Trop Med Hyg, 50(1):52-58, 1994.
Chen et al., “Western immunoblotting analysis of the antibody responses of patients with human monocytotropic ehrlichiosis to different strains ofEhrlichia chaffeensisandEhrlichia canis,” Clin Diag Lab Immunol, 4(6):731-735, 1997.
Dawson et al., “Serologic diagnosis of human ehrlichiosis using twoEhrlichia canisisolates,”J Infect Dis, 163:564-567, 1991.
GenBank Accession No. AAY069965.
GenBank Accession No. AF078553.
GenBank Accession No. AF082744.
GenBank Accession No. AF230642.
GenBank Accession No. U72291.
GenBank Accession No. AAK28699.
GenBank Accession No. AAC68666.
GenBank Accession No. AF078555.
Groves et al., “Transmission ofEhrlichia canisto dogs by ticks (Rhipicephalus sanguineus),”Am J Vet Res, 36:937-970, 1975.
Harrus et al., “Amplification of ehrlichial DNA from dogs 34 months after infection withEhrlichia canis,” J Clin Microbiol, 36(1):73-76, 1998.
Jobling et al., “Analysis of structure and function of the B subunit of cholera toxin by the use of site-directed mutagenesis,”Mol. Microbiol., 5:1755-1767, 1991.
Jongejan et al., “The immunodominant 32-kilodalton protein ofCowdria ruminantiumis conserved within the genusEhrlichia,” Rev Elev Med Vet Pays Trop, 46(1-2):145-152, 1993.
McBride et al., “A conserved, transcriptionally active p28 multigene locus ofEhrlichia canis,” Gene, 254:245-252, 2000.
McBride et al., “Molecular cloning of the gene for a conserved major immunoreactive 28-kilodalton protein ofEhrlichia canis: a potential serodiagnostic antigen,”Clinical and Diagnostic Laboratory Immunobiology, 6(3):392-399, 1999.
McClure, “Mechanism and control of transcription initiation in prokaryotes,”Ann Rev Biochem, 54:171-204, 1985.
Ohashi et al., “Cloning and characterization of multigenes encoding the immunodominant 30-kilodalton major outer membrane proteins ofEhrlichia canisand application of the recombinant protein for serodiagnosis,”Journal of Clinical Microbiology, 36(9):2671-2680, 1998.
Ohashi et al., “Immunodominant major outer membrane proteins ofEhrlichia chaffeensisare encoded by a polymorphic multigene family,”Infect Immun, 66(1):132-139, 1998.
Pharmacia Biotech,BioDirectory, Chapter 9, 217-236, 1996.
Reddy et al., “Molecular characterization of a 28 kDa surface antigen gene family of the tribe Ehrlichiae,”Biochem Biophys Res Comm, 247(3):636-643, 1998.
Rikihisa et al., “Western immunoblot analysis ofEhrlichia chaffeensis, E. canis, or E. ewingiiinfections in dogs and humans,”J Clin Microbiol, 32(9):2107-2112, 1994.
Shankarpappa, “Antigenic and genomic relatedness amongEhrlichia resticii, Ehrlichia sennetsu, andEhrlichia canis,” Int J Syst Bacteriol, 42(1):127-132, 1992.
Storey et al., “Molecular cloning and sequencing of three granulocyticEhrlichiagenes encoding high-molecular-weight immunoreactive proteins,”Infection and Immunity66(4):1356-1363, 1998.
Yu et al., “Characterization of the complete transcriptionally activeEhrlichia chaffeensis28 kDa outer membrane protein multigene family,”Gene, 248:59-68, 2000.
Yu et al., “Detection ofEhrlichia chaffeensishuman tissue by using a species-specific monoclonal antibody,”J. Clin Microbiol. 31:3284-3288, 1993.
National Center for Biotechnology Information, GenBank Accession No. AF078553, GenBank database; Apr. 2, 2001.
National Center for Biotechnology Information, GenBank Accession No. AF078555, GenBank database; Oct. 26, 1998.
National Center for Biotechnology Information, GenBank Accession No. AF0788554, GenBank database; Oct. 26, 1998.
Database EMBL Online, “Ehrlichia canismajor outer membrane protein P30 multigene cluster 1, complete sequence,” XP002346095, Oct. 28, 1998 [abstract].
Database UniProt Online, “P28-2 (Major outer membrane protein P30-10).” XP002346097, Mar. 1, 2001 [abstract].
Database UniProt Online, “P28-9.” XP002346099, Mar. 1, 2001 [abstract].
Database UniProt Online, “P28-3 (Major outer membrane protein P30-4).” XP002346098, Mar. 1, 2001 [abstract].
Database UniProt Online, “P28-1 (Major outer membrane protein P30-5).” XP002346096, Mar. 1, 2001 [abstract].
Database EMBL Online, “Ehrlichia canisp28 multigene locus, partial sequence,” XP002346094, Oct. 20, 1998 [abstract].
Reddy et al., “Molecular Characterization of a 28 kDa Surface Antigen Gene Family of the Tribe Ehrlichiae,”Biochem. Biophys. Res. Comm., 247: 636-643, 1998.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Homologous 28-kilodalton immunodominant protein genes of... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Homologous 28-kilodalton immunodominant protein genes of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Homologous 28-kilodalton immunodominant protein genes of... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3913371

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.