Homogenous method for the detection of a polynucleotide,...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S006120

Reexamination Certificate

active

10203516

ABSTRACT:
The present invention relates to a homogenous method for detecting at least one target polynucleotide in a sample comprising the steps ofcontacting a sample comprising said polynucleotide or polynucleotide forming nucleic acids or derivatives thereof, with labelled oligonucleotide probe under conditions where said oligonucleotide probe selectively hybridizes to said target polynucleotide, wherein the signal of the label increases or decreases upon the hybridization between the oligonucleotide probe and the target polynucleotide,exciting the label, andmonitoring the signal intensity of said label to indicate the presence or absence in a sample of said target polynucleotide.According to the invention, said label is measured by time resolved fluorometry.

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