Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-05-28
1998-09-29
Chin, Christopher L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
422 55, 422 58, 422 8207, 422 8208, 422 8209, 435 71, 435 78, 435 793, 435 794, 435 795, 4352871, 4352872, 4352885, 4352887, 435968, 435808, 436164, 436165, 436172, 436536, 436537, 436800, 436805, 436518, G01N 3353, G01N 33543
Patent
active
058144498
ABSTRACT:
A homogenous affinity assay method, and sensor using such method, for the detection and measurement of analytes that uses an unmodified polyvalent receptor-bearing molecule and having a receptor with at least two binding sites that has an affinity for an analyte of interest, and two groups of molecules wherein one group of molecules is labeled with a molecule capable of generating a measurable response when in close proximity to a label molecule on the other group of molecules. In one embodiment, the groups of molecules also have grafted on them an analog to the analyte of interest. In the absence of the analyte of interest, members of the molecule groups are brought in close proximity to each other through affinity binding to receptor-bearing molecule. This affinity binding results in the formation of a binding complex consisting of members of the first and second groups of molecules and receptors and which results in a response between the labels on the molecules which is capable of measurement. In the presence of the analyte of interest, the binding complexes dissociate due to competitive replacement of the analog with the analyte of interest and affinity binding of the analyte of interest to at least one of the binding sites on the receptors and the labels on the molecules interact in inverse proportion the concentration of the analyte of interest. In another embodiment, the receptor is the analyte of interest and the formation of the binding complexes is a measure of the analyte of interest.
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Ballerstadt Ralph
Schultz Jerome S.
Chin Christopher L.
University of Pittsburgh
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