Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-02-05
1998-12-22
Caputa, Anthony C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 721, 435 74, 435 78, 435 792, 435 793, 435 794, 435 795, 435975, 436500, 436510, 436525, 436526, 436536, 436539, 436544, 436545, 436546, G01N 3353, G01N 33567, G01N 33553, G01N 33536
Patent
active
058517774
ABSTRACT:
The present invention provides methods for detecting the presence of or determining the amount of a ligand in a fluid sample. The methods comprise providing a first reagent comprising a sol particle having a detectable physical property bound to the ligand or ligand analogue (in a competitive format) or a substance capable of specifically coupling with the ligand (in a sandwich format), providing a second reagent having a detectable physical property comprising a sol particle bound to a substance capable of specifically coupling with the ligand and/or ligand analogue, if present, combining the first reagent, second reagent and the fluid sample and detecting before, during or after the reaction, a change in the physical property of the sol particles, which provides a qualitative or quantitative indication of the ligand in the fluid sample. The reagents couple with one another as a function of the presence of the ligand in the sample to thereby produce a change in the physical property of the sol particles which is related to the degree of coupling of the reagents. The invention also provides a kit for detecting the presence of or determining the amount of a ligand in a fluid sample. The invention further provides immunological complexes having a detectable physical property.
REFERENCES:
patent: 3011874 (1961-12-01), Deutsch
patent: 4313734 (1982-02-01), Leuvering
patent: 4366241 (1982-12-01), Tom et al.
patent: 4517288 (1985-05-01), Giegel et al.
patent: 4624923 (1986-11-01), Margel
patent: 4727019 (1988-02-01), Valkirs et al.
patent: 4853335 (1989-08-01), Olsen et al.
patent: 4859612 (1989-08-01), Cole et al.
patent: 4920059 (1990-04-01), Moeremans et al.
patent: 4954452 (1990-09-01), Yost et al.
patent: 4962023 (1990-10-01), Todd et al.
patent: 5079172 (1992-01-01), Hari et al.
patent: 5120643 (1992-06-01), Ching et al.
patent: 5141850 (1992-08-01), Cole et al.
patent: 5202267 (1993-04-01), Ditlow et al.
patent: 5252459 (1993-10-01), Tarcha et al.
patent: 5286452 (1994-02-01), Hansen
patent: 5294369 (1994-03-01), Shigekawa et al.
patent: 5334538 (1994-08-01), Parker et al.
patent: 5403720 (1995-04-01), Sato et al.
patent: 5445971 (1995-08-01), Rohr
patent: 5556756 (1996-09-01), Olsen et al.
patent: 5571726 (1996-11-01), Brooks, Jr. et al.
Leuvering, et al., A Homogeneous Sol Particle Immunoassay for Human Chronic Gonadotrophin Using Monoclonal Antibodies, Journal of Immunological Methods 1983, 60, pp. 9-23.
Leuvering, et al., A Sol Particle Agglutination Assay For Human Chorionic Gonadotrophin, Journal of Immunological Methods 1981, 45, pp.183-194.
M. Brinkley, A Brief Survey of Methods for Preparing Protein Conjugates with Dyes, Haptens, and Cross-Linking Reagents, Bioconjugate Chem. 1992, 3, pp. 2-13.
B. Erlanger, Principles and Methods for the Preparation of Drug Protein Conjugates for Immunological Studies, Pharmacological Reviews 1973, vol. 25, No. 2, pp. 271-280.
Stanworth, et al., Immunochemical analysis of human and rabbit immunoglobulins and their subunits, Handbook of Experimental Immunology 1986, vol. 1: Immunochemistry (Ed. D.M. Weir, Blackwell Scientific Publications) (4th Edition), pp. 12.1-12.46.
P. Parham, Preparation and purification of active fragments from mouse monoclonal antibodies, Handbook of Experimental Immunology 1986, vol. 1: Immunochemistry (Ed. D.M. Weir, Blackwell Scientific Publications) (4th Edition), pp. 14.1-4.23.
Turkevich, et al., A Study of the Nucleation and Growth Processes in the Synthesis of Colloidal Gold, Discussions of the Farady Society 1951, vol. 11, pp. 55-75.
J.E. Beesley, Preparation of Gold Probes, Methods in Molecular Biology 1992, vol. 10, pp. 163-168.
Leuvering, et al., Sol Particle Immunoassay (SPIA), Journal of Immunoassay 1980, 1(1), pp. 77-91.
G. Frens, Controlled Nucleation for the Regulation of the Particle Size in Monodisperse Gold Suspensions, Nature Physical Science 1973, vol. 241, pp. 20-22.
W.D. Geoghegan, et al., Pasive Gold Agglutination, An Alternative To Passive Hemagglutination, Journal of Immunological Methods 1980, 34, pp. 11-21.
J.H.W. Leuvering, et al., A Homogeneous Sol Particle Immunoassay for Total Oestrogens in Urine and Serum Samples, Journal of Immunological Methods 1983, 62, pp. 163-174.
T.C.J. Gribnau, et al., Particle-Labelled Immunoassays: A Review, Journal of Chromatography 1986, 376, pp. 175-189.
H. van Hell, et al., Particle Immunoassays, Alternative Immunoassays 1985, chapter 4, pp. 39-58.
Burgess et al (J. of Cell Biology vol. 111 pp. 2129-2138), Nov. 1990.
Lazar et al (Molecular & Cellular Biology vol. 8 No. 3 pp. 1247-1252), Mar. 1988.
Salgaller et al (Cancer Immunol. Immunother. vol. 39 pp. 105-116, 1994.
Hunter Thomas J.
Pfadenhauer Ernest H.
Caputa Anthony C.
Dade Behring Inc.
Lowen Cara Z.
Navarro Mark
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