Homogeneous polynucleotide displacement assay method kit and rea

Details Homogeneous polynucleotide displacement assay method kit and rea Homogeneous polynucleotide displacement assay method kit and rea

1985-07-17

1989-01-03

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 4, 435 18, 435 19, 435 21, 435810, 436501, 536 26, 536 27, 536 28, 935 77, 935 78, C12Q 168, C12Q 134, C12Q 142

Patent

active

047957018

ABSTRACT:
An RNA signal strand is displaced from a probe strand by a target nucleotide sequence. Without separation, a digestion enzyme selective for displaced RNA, e.g., a polynucleotide phosphorylase, digests the displaced RNA to nucleoside phosphates including ADP. The digestion products are detected, e.g. by phosphorylating the ADP to ATP and detecting the ATP by bioluminescence.

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